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绿鳍马面鲀精子的超微结构
刘 琨,刘长琳,陈四清,张乐乐,边 力
作者单位E-mail
刘 琨 上海海洋大学 水产与生命学院中国水产科学研究院 黄海水产研究所 lkstardust@163.com 
刘长琳 中国水产科学研究院 黄海水产研究所  
陈四清 中国水产科学研究院 黄海水产研究所 chensq@ysfri.ac.cn 
张乐乐 上海海洋大学 水产与生命学院中国水产科学研究院 黄海水产研究所  
边 力 中国水产科学研究院 黄海水产研究所  
摘要:
为探讨绿鳍马面鲀(Thamnaconus septentrionalis)精子的超微结构, 作者应用扫描电镜和透射电镜观察了其精子外部形态与各部位的横切结构。结果表明: 绿鳍马面鲀精子由头部和尾部两部分组成。精子头部呈卵圆形, 无顶体, 头部主要结构是细胞核, 染色质致密, 核内存在少量染色质空隙, 无液泡;核后端正中有凹入核内的植入窝, 呈棒状, 植入窝较深, 最深处占细胞核纵长的5/6, 最宽处在细胞核末端, 约占头部宽度的1/3。中心粒复合体位于植入窝内, 由近端中心粒、中心粒间体和基体组成, 近端中心粒的长轴与基体的纵轴垂直, 排列方式为“T”型; 头部末端为袖套, 内含5个或6个均匀分布的线粒体球和少量随机分布的囊泡。尾部细长, 自基体向后伸出, 尾部无侧鳍结构, 其核心结构是轴丝,轴丝为典型的“9+2”微管复合体结构。揭示其精子的形态结构, 以期丰富硬骨鱼类繁殖生理学内容, 同时为鱼种鉴别提供依据。
关键词:  绿鳍马面鲀(Thamnaconus septentrionalis)  精子  超微结构
DOI:10.11759/hykx20170119003
分类号:
基金项目:中国水产科学研究院黄海水产研究所基本科研业务(20603022016005)
Ultrastructure of spermatozoa of Thamnaconus septentrionalis
LIU Kun,LIU Chang-lin,CHEN Si-qing,ZHANG Le-le,BIAN Li
Abstract:
To study the ultrastructure of the spermatozoa of Thamnaconus septentrionalis, we observed its external form and cross-section structure by scanning and transmission electron microscopy. The spermatozoa of Thamnaconus septentrional consists of a head and tail. The head has no acrosome, is shell-shaped, and its main structure is the nucleus. At its caudal end there is a deep implantation fossa, 5/6 as deep as the head length. We observed the nucleus to consist of electron dense chromatin materials, with a head pit and nuclear vacuole whose chromatin was free. The centriolar compound of the midpiece is situated in the implantation fossa, which consists of three parts: the proximal centriole, the intercentriolar, and the basal body. The proximal centriole and the basal body were arranged orthogonally in a “T” structure, whereas the basal body was parallel to the spermatozoa. The sleeve was very deep, with the deepest point one third of the head length. The implantation fossa are shaped like rods and the sleeve is located behind the nucleus, which contains five to six mitochondria arranged in a circle. We found there to be a few ellipse vesicles in the sleeve outside the mitochondrion. The structure of the flagellum was such that it facilitated spermatozoa movement. The main structure of the tail was the axoneme, which was slim and long. The tail structure was a typical “9 + 2” model. The flagella were fine and they had no lateral fins. In this paper, we describe the morphological structure of the spermatozoa to enrich our understanding of the physiological contents of teleosts and to provide the basis for the identification of fish species.
Key words:  Thamnaconus septentrionalis  spermatozoa  ultrastructure
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