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条纹斑竹鲨(Chiloscyllium plagiosum)5个地理群体的简单重复序列中间区域分析
潘滢,郑利兵,王军
厦门大学海洋与地球学院
摘要:
简单重复序列中间区域(inter-simple sequence repeat,ISSR)是一种微卫星类分子标记。为明确条纹斑竹鲨(Chiloscyllium plagiosum)中国群体的遗传多样性和遗传关系,本文作者利用ISSR技术对福建厦门(XM)、福建平潭(PT)、广东湛江(ZJ)、海南海口(HK)和台湾(TW)5个土著地理群体的条纹斑竹鲨进行了遗传多样性和遗传关系分析。从56对ISSR引物筛选出13对多态性引物,扩增共获得81个重复性的位点,其中多态性位点57个,多态位点百分率为70.37%。5个群体的多态位点百分率、Nei’s基因多样性和Shannon’s信息指数分别为38.27%~58.02%,0.1353~0.2155和0.2032~0.3193。UPGMA聚类分析表明PT群体首先和XM群体聚类,再与TW群体聚类,最后与ZJ群体和HK群体形成的分支聚类,即形成了与地理距离远近相关的基因交流模式。
关键词:  条纹斑竹鲨  ISSR  遗传多样性  地理群体
DOI:
分类号:Q178.53
基金项目:国家自然科学基金(40876080)[Foundation: National Natural Science Foundation of China, No. 40876080]
Genetic Structure Analysis on Five Populations of Chiloscyllum plagiosum by Inter-simple Sequence Repeat Molecular Marker
PAN Ying1,2, ZHENG Libing1,2, WANG Jun1,2
1.College of Ocean Earth Science,Xiamen University;2.China
Abstract:
Inter-simple sequence repeat (ISSR) is a molecular marker of microsatellite. In the present study, the genetic diversity and genetic relationship analysis from five native populations of the white spotted bamboo shark Chiloscyllium plagiosum sampled from Xiamen(XM)and Pingtan (PT) of Fujian, Zhanjiang (ZJ)of Guangdong, Haikou (HK) of Hainan and Taiwan (TW) were carried out by ISSR technique. 13 polymorphic primers were selected from 56 ISSR primers. 81 locus with great reproducibility, including 57 polymorphic locus were acquired by PCR amplification, indicating that the percentage of polymorphic loci was 70.37%. The polymorphic loci percentage , the Shannon diversity indices and the Nei’s gene diversity value in 5 populations were 38.27%~58.02%, 0.1353~0.2155 and 0.2032~0.3193 respectively. The UPGMA tree constructed by MEGA5.0 showed that PT and XM populations clustered together firstly, then clustered with TW population, and finally joined to the branch clustered by ZJ and HK populations, which formed the gene exchange pattern correlated to geographical distance.
Key words:  Chiloscyllium plagiosum  Inter-simple sequence repeat (ISSR)  genetic diversity  geographical populations
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