| 摘要: |
| 历史耳石、鳞片作为鱼类群体遗传与进化研究宝贵的DNA来源，记录了几十年甚至几百年的遗传信息，使得鱼类群体遗传与进化研究可以扩展到历史时间尺度，从而可以直接反映人类活动对鱼类遗传多样性及适应性进化的影响。近十几年来，随着高通量测序技术的蓬勃发展，历史耳石、鳞片作为基因组数据的来源成为现实，可提供更为精细的遗传数据。然而，历史样本具有DNA高度降解、外源DNA污染等特点，利用历史DNA进行群体遗传学研究仍然是一项十分困难的工作。本文对历史耳石、鳞片样本DNA的提取方法，基因分型技术进行了总结，以期为克服这些困难提供思路。另外，本文综述了利用历史样本进行鱼类群体遗传与进化研究的现状与热点。伴随新一代分子技术的普及与发展，历史DNA可以提供更为丰富的遗传信息，将为鱼类群体遗传与进化相关研究提供新的思路与证据，未来势必拥有更广阔的应用前景。 |
| 关键词: 耳石，鳞片，历史DNA，群体遗传，进化 |
| DOI： |
| 分类号: |
| 基金项目: |
|
| Application of historical DNA in studies of fish population genetics and evolution |
|
Liu Jin-Xian,Xu Zhe
|
|
Institute of Oceanology, Chinese Academy of Sciences
|
| Abstract: |
| Historical otoliths and scales constitute a valuable source of DNA record genetic information for decades and even centuries, which potentially enables extension of the temporal scale of genetic and evolution studies of fish populations. Thus, historical DNA could directly reflects influence of human activities on genetic diversity and adaptive evolution of fish. In recent decades, with the advances in high-throughput sequencing technologies, historical otoliths and scales have been used used as a source of genomic data, which provide more refined genetic data. Nevertheless, population genetic studies with historical DNA are still challenging. This is primarily due to the high DNA degradation and exogenous DNA contamination of historical DNA. Here, we reviewed the DNA extraction protocols and genotyping technologies of historical otoliths and scales which could help researchers to plan their research using historical specimens. In addition, we reviewed hot topics in the fish population genetics and evolution with historical samples. Increasing accessibility to new technologies will provide researchers more abundant genetic information from historical DNA. As an effective tool, historical DNA will provide new ideas and evidence for the study of fish population genetics and evolution, which will certainly have a broader application in the future. |
| Key words: otolith scale historical DNA population genetics evolution |