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引用本文:鞠豪,张全斌.絮凝剂-树脂联合法从琼胶中制备琼脂糖工艺研究[J].海洋科学,2020,44(9):139-145.
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絮凝剂-树脂联合法从琼胶中制备琼脂糖工艺研究
鞠豪1,2,3, 张全斌1,3
1.中国科学院海洋研究所 实验海洋生物学重点实验室, 山东 青岛 266071;2.中国科学院大学, 北京 100049;3.中国科学院 海洋大科学研究中心, 山东 青岛 266071
摘要:
琼脂糖是一种具有高凝胶特性的天然高分子红藻多糖,具有良好的物理、化学和热稳定性,在医学和科研方面具有广泛的用途。在琼脂糖制备工业中,传统生产技术的局限性造成了国内琼脂糖尚未规模化生产。因此,有必要开发新的成本可控的琼脂糖制备方法。本文采用一种聚丙烯酰胺和弱碱性苯丙烯系阴离子交换树脂联用的方法来分离制备琼脂糖,以江蓠琼胶为原料,通过单因素试验优化琼脂糖的制备参数并对琼脂糖的理化指标进行测定。所得琼脂糖硫酸根含量为0.22%、凝胶强度为1 524 g/cm2、透明度为58.9%,凝固温度为39.7℃、融化温度为87.6℃,表明其具有相对较高的质量。该工艺简单经济,对发展琼脂糖工业具有一定的参考价值。
关键词:  琼胶  聚丙烯酰胺  弱碱性苯丙烯系阴离子交换树脂  琼脂糖
DOI:10.17759/hykx20200323001
分类号:Q539+.9
基金项目:国家重点研发计划(2019YFD0900705),福建省科技计划项目(2017T3013),江苏省创新企业家人才团队计划
Flocculant-resin combined method for preparation of agarose from agar
JU Hao1,2,3, ZHANG Quan-bin1,3
1.Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;2.University of Chinese Academy of Sciences, Beijing 100049, China;3.Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao 266071, China
Abstract:
Agarose is a natural macromolecular red algae polysaccharide with high gel characteristics. It has good physical, chemical and thermal stability, and has a wide range of uses in medicine and scientific research. The limitation of traditional production technology has made it impossible to produce agarose on a large scale in China. Therefore, it is necessary to develop new cost-controllable agarose preparation methods. In this paper, a method of polyacrylamide and anion exchange resin was used to separate and prepare agarose. Gracilaria agar was used as raw material. The agarose preparation parameters were optimized by single-factor experiments and the physical and chemical indexes of agarose were determined. The obtained agarose sulfate content was 0.22%, the gel strength was 1 524 g/cm2, the transparency was 58.9%, the solidification temperature was 39.7℃, and the melting temperature was 87.6℃, indicating that it had a relatively high quality. The process is simple and economical, and has certain reference value for the development of the agarose industry.
Key words:  agar  polyacrylamide  anion exchange resin  agarose
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