| 摘要: |
| 为探究多环芳烃(PAHs)对海洋生物的生态毒理效应，将仿刺参(Apostichopus japonicus)分别暴露于浓度为10、20、50、100、150 μg·L-1的菲、三甲基菲、蒽和二甲基蒽中，检测4种代表性多环芳烃胁迫下仿刺参的耗氧率和排氨率。结果显示：在不同浓度多环芳烃的胁迫下，仿刺参的耗氧率均显著高于对照组（P<0.05），且单位体重仿刺参耗氧率受三甲基菲浓度影响最大，其相关方程为y = 4.8971x + 1.2267，R2 = 0.8945；单位体重仿刺参排氨率随4种多环芳烃浓度的增加出现上下波动的现象，且在菲胁迫下，其排氨率波动幅度最大，在菲浓度为20 μg·L-1时达到最大值22.87 μg·g-1·h-1；O:N比在4种不同浓度多环芳烃的作用下均显著高于对照组并整体随暴露浓度呈上升趋势（P<0.05），且受二甲基蒽浓度影响最大，其相关方程为y = 0.3471x + 0.3967，R2 = 0.8435。上述实验结果为多环芳烃类污染物对仿刺参的毒害作用机制探究提供了科学依据。 |
| 关键词: 多环芳烃，仿刺参，耗氧率，排氨率，O:N比 |
| DOI： |
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| Effects of Four PAHs on 0xygen Consumption and Ammonia Exhalation Rate of Apostichopus japonicus |
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Liushuai
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Ocean University of China
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| Abstract: |
| To investigate the toxic effects of PAHs mainly composed of phenanthrene, 3-methylphenanthrene, anthracene and 2-methylanthracene on Marine organisms, Apostichopus japonicus was exposed to them at concentrations of 10, 20, 50, 100 and 150 μg·L-1, respectively. The oxygen consumption rate and ammonia excretion rate of A. japonicus under the stress of four representative PAHs were measured. The results showed that under the stress of four different concentrations of PAHs, the oxygen consumption rate of A. japonicus was significantly higher than that of the control group (P<0.05), and the concentration of 3-methylphenanthrene had the greatest effect on the oxygen consumption rate per unit body weight, and the correlation equation was y = 4.8971x + 1.2267, R2 = 0.8945; Ammonia emission per unit body weight fluctuated up and down with the increase of PAHs concentration. Under phenanthrene stress, the ammonia emission rate fluctuated the most, and reached the maximum of 22.87 μg·g-1·h-1 when phenanthrene concentration was 20 μg·L-1; The O:N ratio were significantly higher than those of the control group under the action of four different concentrations of PAHs, and increased with the overall exposure concentration (P<0.05), and was most affected by the concentration of 2-methylanthracene, the correlation equation is y = 0.3471x + 0.3967, R2 = 0.8435. The above experimental results provide a scientific basis for exploring the toxic mechanism of PAHs on A. japonicus. |
| Key words: PAHs ?Apostichopus japonicus ?oxygen consumption ammonia excretion O:N ratio |