| 摘要: |
| 为有效抑制萱藻种质保存和丝状体扩增时膨胀色球藻的污染，本实验应用实验生态学法，研究了恩诺沙星、磺胺嘧啶和呋喃西林3种抗菌药对共培养的萱藻丝状体和膨胀色球藻生长的影响。结果显示：在5~100 mg/L范围内，50 mg/L恩诺沙星适于除去萱藻种质保存或丝状体扩增过程中出现的膨胀色球藻，实验第18 d膨胀色球藻的日均增长率为-2.67%，萱藻丝状体的单室孢子囊发育良好；在10~150 mg/L范围内，100 mg/L磺胺嘧啶能有效抑制膨胀色球藻的生长，实验第18 d膨胀色球藻的日均增长率为-0.58%，萱藻丝状体日均增长率为2.91%，单室孢子囊发育良好；150 mg/L磺胺嘧啶会使萱藻丝状体的部分细胞呈浅褐色，单室孢子囊发育不良；在1~20 mg/L范围内，20 mg/L呋喃西林能抑制膨胀色球藻的生长，实验第18 d膨胀色球藻日均增长率为-0.78%，但同时影响了萱藻丝状体的正常生长，单室孢子囊发育不良。实验证明，50 mg/L恩诺沙星和100 mg/L磺胺嘧啶最适于解决萱藻种质保存和丝状体扩增过程中膨胀色球藻的污染。 |
| 关键词: 萱藻 丝状体 膨胀色球藻 共培养 抗菌药 |
| DOI：10.11759/hykx20201012002 |
| 分类号:Q14 |
| 基金项目:山东省重点研发计划（2016GSF115042） |
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| Effects of enrofloxacin, sulfadiazine and nitrofurazone on the growth of filaments of Scytosiphon lomentaria and Chroococcus turgidus in the co-cultured condition |
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LIU De-ju, ZHANG Min, ZHUANG Ying-rui, BI Meng, GONG Xiang-zhong
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College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China
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| Abstract: |
| In order to inhibit the growth of Chroococcus turgidus during the germplasm preservation and the amplification of filaments of Scytosiphon lomentaria, effects of enrofloxacin, sulfadiazine and nitrofurazone on the growth of filaments of S. lomentaria and C. turgidus in the co-cultured condition are studied by experimental ecology methods. Results indicated that:①In the range of 5~100mg/L, 50mg/L enrofloxacin is suitable for inhibiting C.turgidus from the germplasm preservation and the amplification of filaments of S.lomentaria. The average daily growth rate of C.turgidus is -2.67% and the development of unilocular sporangia of filaments of S.lomentaria is well on the 18th day; ②In the range of 10~150mg/L, 100mg/L is the optimum concentration of sulfadiazine to inhibit the growth of C.turgidus. On the 18th day, the average daily growth rate of C.turgidus is -0.58%, the average daily growth rate of S.lomentaria is 2.91% and the development of unilocular sporangia of filaments of S.lomentaria is well; The growth of filaments of S.lomentaria is in bad condition and unilocular sporangia was poorly developed at 150mg/L; ③In the range of 1~20 mg/L, 20 mg/L nitrofurazone has the inhibitory effect on C.turgidus and the filaments of S.lomentaria, the average daily growth rate of C.turgidus is -0.78% on the 18th day, and at the same time, the normal growth of filaments of S.lomentaria also be affect, and the unilocular sporangia developed poorly. All this experiments have proved that:50mg/L enrofloxacin and 100mg/L sulfadiazine are suitable for solving the contamination of C.turgidus during the germplasm preservation and amplification of filaments of Scytosiphon lomentaria. |
| Key words: Scytosiphon lomentaria filaments Chroococcus turgidus co-culture antimicrobial |
