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东海几种鳗鲡目鱼类DNA条形码研究
宁子君1, 刘玉萍2, 高天翔2, 蒋日进3, 杨天燕1
1.浙江海洋大学;2.浙江海洋大学水产学院;3.浙江省海洋水产研究所
摘要:
本研究采集了分布于我国东海的前肛鳗(Dysomma anguillaris)、短尾蛇鳗(Ophichthus brevicaudatus)、艾氏蛇鳗(Ophichthus evermanni)、海鳗(Muraenesox cinereus)、黑尾吻鳗(Rhynchoconger ectenurus)、微鳍新鳗(Neenchelys parvipectorali)、大头蚓鳗(Moringua macrocephalus)、梅氏美体鳗(Ariosoma meeki)和星康吉鳗(Conger myriaster)9种鳗鲡目鱼类,采用PCR技术扩增了线粒体COI基因片段序列,结合Genbank数据库中下载的5种鳗鲡目鱼类同源序列,分析比较了序列组成和差异,并以光海鳝Muraena argus和细点海鳝Muraena augusti为外群,基于最大似然法构建了鳗鲡目中6科11属14种鱼类的系统发育树,探讨了该类群鱼类的系统进化关系。结果显示:72条序列共检测到43种单倍型,4种碱基含量分别为27.4 %(T)、28.2%(C)、25.8 %(A)、18.6%(G),平均A+T含量(53.2%)高于G+C含量(46.8%),表现出明显的碱基组成偏向性。基于K2P(Kimura 2-parameter)模型计算得出不同种间的平均遗传距离为0.2188,不同属间的平均遗传距离为0.2308,不同科间的平均遗传距离为0.2327,分类阶元越高,遗传距离越大。系统进化树显示蛇鳗科物种都能够形成独立的分支,并得到有效的区分,而其他类群存在混杂现象。以上结果表明,由于鳗鲡目鱼类种类多且分布广,线粒体COI基因只适用于较低分类阶元(如科内属间、属内种间)间的物种鉴定,该类群鱼类系统发育关系还有待于结合多种DNA条形码进行深入探讨。
关键词:  鳗鲡目  COI基因  DNA条形码  遗传距离  系统发育
DOI:
分类号:
基金项目:
DNA barcoding analysis of some Anguilliformes fishes in the East China Sea
Ning Zijun1, Liu Yuping2, Gao Tianxiang2, Jiang Rijin3, Yang Tianyan1
1.Zhejiang Ocean University;2.Fishery College,Zhejiang Ocean University,Zhejiang Zhoushan;3.Zhejiang Marine Fisheries Research Institute,Zhejiang Zhoushan
Abstract:
In this study, nine species of Anguilliformes were collected from the East China Sea, including Dysomma anguillaris, Ophichthus brevicaudatus, O. evermanni, Muraenesox cinereus, Rhynchoconger ectenurus, Neenchelys parvipectorali, Moringua macrocephalus, Ariosoma meeki and Conger myriaster. The mitochondrial COI gene fragments were amplified by PCR and sequenced. The homologous sequences of five anguiliformes downloaded from Genbank database were analyzed and compared. The maximum likelihood phylogenetic tree of 14 species, 11 genera and 6 families in Anguilliformes was constructed with Muraena argus and Muraena augusti as outgroups. The results showed that a total of 43 haplotypes were detected in 72 sequences, and the contents of 4 kinds of bases were 27.4% (T), 28.2% (C), 25.8% (A) and 18.6% (G), respectively. The average content of A+T (53.2%) was higher than that of G+C (46.8%), showing obvious bias of base composition. Based on the Kimura 2-parameter (K2P) model, the average genetic distances between species, genera and families were 0.2188, 0.2308 and 0.2327, respectively, indicating the higher the taxonomic order, the greater the genetic distance. The phylogenetic tree showed that Ophichthyidae species can form independent clade and be effectively distinguished. However, the clustering of other genera and families produced a mixed phenomenon. The results suggested that because of species diversity and wide distribution of Anguilliformes fishes, mitochondrial COI gene might be more suitable for species identification and classification in lower taxonomic category (such as coordinal and congeneric), and the phylogenetic relationships of Anguilliformes species need to be further studied in combination with multiple DNA barcodes.
Key words:  Anguilliformes  COI gene  DNA barcoding  genetic distance  phylogeny
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