摘要: |
为探究长牡蛎在繁殖期间的糖原含量与类胰岛素基因(cgMIP123、cgMIP4、cgMILP7、cgILP)和相关转录调控因子(cgPdx)相对表达量的相关性,自2020年5月至2020年10月采集了山东胶南养殖海区的长牡蛎,测定了血糖含量、糖原含量、条件指数、类胰岛素基因相对表达量及环境因子(酸度、温度、盐度)等数据,采用多元统计方法对数据进行分析。logistic回归分析结果显示,长牡蛎配子排放前后血糖含量和内脏团糖原含量具有显著差异。构建的回归模型可以通过血糖含量和内脏团糖原含量准确判断配子是否已经排放,区分度C-index为0.903,Hosmer-Lemeshow拟合优度检验χ2值为9.06,P>0.05,验证结果显示该模型可靠。多元线性回归分析结果显示:条件指数与cgMIP123和cgMIP4基因的相对表达量具有相关性,R2为0.91,P值为0.0076,极显著相关;内脏团和唇瓣组织的糖原含量与ILPs和cgPdx相对表达量具有一定的相关性,其中cgMILP7的相对表达量与内脏团和唇瓣组织的糖原含量呈负相关,cgPdx相对表达量与唇瓣组织的糖原含量呈负相关,cgMIP4和cgILP的相对表达量与糖原含量呈正相关。 |
关键词: 长牡蛎 血糖 类胰岛素 胰十二指肠同源异型盒基因 多元统计 |
DOI:10.11759/hykx20210304002 |
分类号:S917.4 |
基金项目:国家自然科学基金(41776152);中国科学院海洋大科学研究中心重点部署项目(COMS2019Q11);青岛海洋科学与技术试点国家实验室开放基金(QNLM2016ORP0408) |
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Glycogen content during reproduction seasons of Pacific oyster and the correlation analysis with relative expression of insulin-like genes |
MIAO Xiao-jing1,2, DENG Shao-xi1,2, LI Liang-heng1, XU Fei1,3
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1.Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;2.University of Chinese Academy of Sciences, Beijing 100049, China;3.Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao 266071, China
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Abstract: |
In the present study, glycogen content variations during the reproduction period of Crassostrea gigas (Pacific oyster) and their correlations with the expression patterns of insulin-like genes (cgMIP123, cgMIP4, cgMILP7, cgILP) and the related transcription factor (cgPdx) were investigated. The blood glucose and glycogen content, condition index, and relative gene expression level were assayed on oysters cultured on a farm in Jiaonan, Shandong. Furthermore, marine environmental factors such as acidity, temperature, and salinity were collected from May to October 2020. Multivariate logistic regression analysis indicated a high correlation between the spawning and variations of blood glucose and glycogen content around the pyloric process. A regression model was constructed to estimate the gonad discharging status by the two parameters. The concordance index was at 0.903, while the χ2 value was at 9.06 (P > 0.05) for the Hosmer-Lemeshow test, indicating a good fit. Application of the model to verification samples also showed high reliability. The multiple linear regression analysis exhibited correlations of growth or physiological values with the expression pattern of some genes: the condition index was correlated with cgMIP123 and cgMIP4 (R2: 0.91, P: 0.0076); the glycogen content around the pyloric process and labial palps showed correlation with cgILPs and cgPdx; the relative expression of cgMILP7 was negatively correlated with the glycogen content of all the assayed tissues; cgPdx was negatively correlated with the glycogen content of labial palps; cgMIP4 and cgILP were positively correlated with glycogen content. |
Key words: Crassostrea gigas Pacific oyster blood glucose content insulin-like genes Pdx multivariate statistics |