| 引用本文: | 戴雪田,刘冬妮,杨芷,李建磊,钱伟,朱小山.纳米二氧化钛对海洋硅藻有机磷酸酯累积、转化及毒性的影响[J].海洋科学,2025,49(4):25-38. |
| |
|
| |
|
|
| 本文已被：浏览 246次 下载 100次 |
码上扫一扫！ |
|
|
| 纳米二氧化钛对海洋硅藻有机磷酸酯累积、转化及毒性的影响 |
|
戴雪田1, 刘冬妮2,3, 杨芷4, 李建磊1, 钱伟1, 朱小山1
|
|
1.海南大学 生态学院, 海南 海口 570228;2.海南省检验检测研究院食品检验检测中心, 海南 海口 570000;3.国家市场监督管理总局重点实验室(热带果蔬质量与安全), 海南 海口 570000;4.海南大学 环境科学与工程学院, 海南 海口 570228
|
|
| 摘要: |
| 金属纳米颗粒在现代工业和日常生活中得到广泛应用, 其对生态系统产生的潜在风险受到人们的广泛关注。然而, 目前有关金属纳米颗粒对共存污染物的生物转化研究相对较少。本研究以海洋硅藻三角褐指藻(Phaeodactylum tricornutum)为研究对象, 探究纳米二氧化钛(nano-titanium dioxide, nTiO2)对有机磷酸酯(organophosphate esters, OPEs)的累积、转化及毒性影响。通过OPEs的急性毒性实验、OPEs的单独以及与nTiO2的共暴露实验发现, 磷酸三苯酯(triphenyl phosphate, TPHP)毒性显著高于其他OPEs, 其主要代谢产物磷酸二苯酯(diphenyl phthalate, DPHP)毒性显著降低, 表明OPEs的生物转化具有解毒作用。然而, 当nTiO2存在时, 藻体内的TPHP质量浓度显著升高, DPHP质量浓度显著下降, 表明nTiO2的存在显著抑制了TPHP向DPHP的转化, 导致TPHP的累积增加并显著增强其对藻体的毒性反应。这种毒性增强效应表现为加剧了藻体的氧化应激, 包括超氧化物歧化酶(SOD)活性上升和丙二醛(MDA)含量显著增加。进一步的机制研究表明, nTiO2通过促进OPEs在藻表面的吸附富集并抑制CYP450酶基因的表达, 干扰了OPEs的代谢过程, 从而增强了其毒性效应。本研究揭示了nTiO2对OPEs生物转化的抑制作用及其增强毒性的潜在机制, 为海洋环境中纳米颗粒与有机污染物的相互作用提供了新的理论依据, 并为环境风险评估提供了关键数据支持。 |
| 关键词: 三角褐指藻|nTiO2|OPEs|生物转化|机制 |
| DOI：10.11759/hykx20241209001 |
| 分类号:X55 |
| 基金项目:国家自然科学基金(42407382);海南省自然科学基金面上项目(323MS011);海南大学生态文明协同创新中心项目(XTCX2022STC20);海南省科技人才创新项目(KJRC2023C11) |
|
| Effects of titanium dioxide nanoparticles on the accumulation, transformation, and toxicity of organophosphate esters in marine diatoms |
|
DAI Xuetian,LIU Dongni,YANG Zhi,LI Jianlei,QIAN Wei,ZHU Xiaoshan
|
|
1.School of Ecology, Hainan University, Haikou 570228, China;2.Hainan Provincial Institute of Quality and Testing Food Testing Center, Haikou 570000, China;3.Key Laboratory of Tropical Fruits and Vegetables Quality and Safety, State Administration for Market Regulation, Haikou 570000, China, 4. School of Environmental Science and Engineering, Hainan University, Haikou 570228, China
|
| Abstract: |
| Metal-based nanoparticles (MNPs) are extensively employed in modern industry and everyday life, raising substantial concerns about their potential risks to ecosystems. However, research on how MNPs influence the biotransformation of coexisting pollutants remains limited. This study explores the effects of nano-titanium dioxide (nTiO2) on the accumulation, transformation, and toxicity of organophosphate esters (OPEs) in the marine diatom Phaeodactylum tricornutum. Through acute toxicity assays, single-exposure experiments, and co-exposure experiments with nTiO2, it was observed that triphenyl phosphate (TPHP) exhibited significantly greater toxicity than other tested OPEs. In contrast, its primary metabolite, diphenyl phosphate (DPHP), showed markedly reduced toxicity, indicating a detoxification effect resulting from OPE biotransformation. However, the presence of nTiO2 significantly increased the intracellular accumulation of TPHP while reducing DPHP levels, suggesting that nTiO2 inhibits the conversion of TPHP to DPHP. This inhibition leads to greater TPHP retention and elevated toxicity in algae. The increased toxicity was reflected in elevated oxidative stress, as evidenced by a significant rise in superoxide dismutase (SOD) activity and malondialdehyde (MDA) content. Further mechanistic investigations revealed that nTiO2 enhances the adsorption and enrichment of OPEs on the algal surface while inhibiting CYP450 gene expression, thereby impairing OPE metabolism and intensifying their toxic effects. This study elucidates the inhibitory role of nTiO2 in the biotransformation of OPEs and its contribution to heightened toxicity. It offers new theoretical insights into the interactions between nanoparticles and organic pollutants in marine environments and provides critical data for environmental risk assessment. |
| Key words: Phaeodactylum tricornutum|titanium dioxide nanoparticles|organophosphate esters|biotransformation|mechanism |
|
|
|
|
|
|