| 引用本文: | 张建敏,史博,王静,阙华勇,黄小青.两个海区福建牡蛎糖原相对高低群体代谢组差异分析[J].海洋科学,2026,50(2):37-50. |
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| 两个海区福建牡蛎糖原相对高低群体代谢组差异分析 |
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张建敏1,2,3, 史博1,2,3, 王静4, 阙华勇1,2,3, 黄小青4
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1.集美大学水产学院, 福建 厦门 361021;2.海水养殖生物育种全国重点实验室, 福建 厦门 361021;3.农业农村部东海海水健康养殖重点实验室, 福建 厦门 361021;4.佛山市海天(高明)调味食品有限公司, 广东 佛山 528000
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| 摘要: |
| 牡蛎是我国产量最高的海水养殖贝类, 2024年全国养殖产量高达725.25万吨, 其中福建牡蛎(Crassostrea angulata)是福建地区的主要养殖品种。糖原含量是牡蛎重要的品质性状, 可以显著影响其风味。目前关于福建牡蛎糖原含量调控机制的研究十分有限, 限制了相关遗传改良工作的开展。本研究将同一群体福建牡蛎在莆田湄洲和泉州石井两个海区中进行养殖, 并分别对每个海区中存在显著糖原含量差异的个体进行代谢组学分析。结果显示: 养殖10个月后, 两个海区福建牡蛎糖原含量均存在极显著个体差异, 最大差异倍数分别为17.42和8.79倍。低糖原组与高糖原组相比, 两个海区分别鉴定出189和361个差异代谢物, 共同富集的代谢通路有花生四烯酸代谢、神经活体配体-受体相互作用及FOXO信号; 湄洲海区中富集到了D-氨基酸代谢通路, 石井海区中则富集到甘油磷脂代谢通路。福建牡蛎可能通过多种途径影响体内糖原含量, 如上调LXA4表达, 抑制炎症反应并减弱机体能量消耗, 促进糖原储存; 通过D-氨基酸代谢通路、淀粉与蔗糖代谢通路强化糖异生等过程产生更多的葡萄糖, 用于合成糖原等。本研究为后续福建牡蛎糖原性状的遗传改良提供基础资料。 |
| 关键词: 福建牡蛎 糖原含量 个体差异 非靶向代谢组学 |
| DOI：10.11759/hykx20251218002 |
| 分类号:S968.31 |
| 基金项目:国家自然科学基金项目(32172979); 漳州诏安水产养殖加工产业链科技特派团(2024N0057) |
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| Metabolomic differences of Crassostrea angulata populations from two seas in Fujian based on relatively high and low glycogen contents |
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Zhang Jianmin1,2,3, Shi Bo1,2,3, Wang Jing4, Que Huayong1,2,3, Huang Xiaoqing4
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1.College of Fisheries, Jimei University, Xiamen 361021, China;2.National Key Laboratory of Mariculture Biobreeding, Xiamen 361021, China;3.Key Laboratory of Healthy Mariculture in the East China Sea, Ministry of Agriculture and Rural Affairs, Xiamen 361021, China;4.Foshan Haitian (Gaoming) Condiment Food Co., Ltd., Foshan 528000, China
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| Abstract: |
| Oysters are the most productive mariculture shellfish in China, with the national aquaculture output reaching 7, 252, 500 tons in 2024. Among them, Crassostrea angulata is the main species cultured in Fujian Province. Glycogen content is a crucial, quality-associated trait that considerably affects oyster flavor. However, research on the mechanisms regulating glycogen content in C. angulata is limited, hindering progress in genetic improvement. In this study, C. angulata from a single population were cultured in two sea areas, Meizhou (Putian) and Shijing (Quanzhou). Metabolomic analysis was performed on individuals from each area with notable variations in glycogen content. After 10 months of culture, these differences were highly notable, with the maximum variations being in the multiples of 17.42 and 8.79, respectively. Compared with the high-glycogen group, 189 and 361 differentially accumulated metabolites were identified in the low-glycogen groups from the two sea areas, respectively. The commonly enriched metabolic pathways included arachidonic acid metabolism, neuroactive ligand-receptor interaction, and the FOXO signaling pathway. In addition, the D-amino acid metabolism pathway was enriched in the specimens from Meizhou, while the glycerophospholipid metabolism pathway was enriched in the specimens from Shijing. C. angulata may affect glycogen content through multiple pathways: upregulating the expression of LXA4 to inhibit inflammatory response, reducing energy consumption, and promoting glycogen storage, enhancing gluconeogenesis and other processes through the D-amino acid and starch-sucrose metabolism pathways to produce more glucose for glycogen biosynthesis. This study provides basic data for the subsequent genetic improvement of glycogen-related traits in C. angulata. |
| Key words: Crassostrea angulata glycogen content individual difference nontargeted metabonomics |
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