| 摘要: |
| 随着抗菌药物在水产养殖中用量的增加及混合使用现象的出现，水产病原菌表现出更强的耐药性甚至是多重耐药性，大大增加了水产养殖中细菌性病害防治的难度。细菌耐药性最常见的传递方式是耐药质粒的转移。本研究通过对多个水产致病弧菌质粒上新霉素及卡那霉素的耐药基因aph(3’)-IIa进行检测分析，并利用电穿孔法将筛选得到的3个受试菌株上的质粒分别转入大肠杆菌中，结果从3个转化子中检测到目的基因。进一步的药敏测试结果显示，3个转化子的最小抑菌浓度（MIC值）以及最小杀菌浓度（MBC值）均明显上升。可见，在特定条件下，水生致病性弧菌对氨基糖苷类（新霉素和卡那霉素）的耐药质粒可转移至大肠杆菌并参与介导其耐药性，大大降低了受体菌对新霉素和卡那霉素的敏感性。本研究将为环境弧菌与临床细菌对氨基糖苷类药物的抗性传递研究提供数据基础和参考依据。 |
| 关键词: 致病弧菌 耐药质粒 新霉素 卡那霉素 耐药机制 |
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| Transmission mechanism of resistance to aminoglycoside for pathogenic marine Vibrio spp. |
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wangruixuan1, WANGJIANGYONG2, LIYUNPING3, GUOZIHAN4, LIBIN2, LIYUN1, ZHUHUI1
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1.Hanshan Normal University;2.South China sea fisheries research institute;3.South China Normal University;4.California Baptist university
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| Abstract: |
| The increasing amount of antimicrobial agents in aquaculture and the emergence of the multi-antibiotics use have lead to serious antibiotic-resistances even the multi-resistance, which had increased the difficulty of prevention and control for bacterial diseases in aquaculture. The most important way for bacteria to transmit antibiotic-resistance is the transfer of resistant plasmids. At the present study, aminolycoside resistant gene aph(3’)-IIa located on plasmid DNA was amplified by polymerase chain reaction (PCR), then three resistant plasmids were sequenced and transformed into Escherichia coli respectively by electroporation. It was showed that the target gene sequence was detected from 3 mutants. The result of resistance analysis suggested that the minimal inhibitory concentration (MIC) and the minimal bactericidal concentration (MBC) three transformants were all much higher than the MIC and the MBC of standard strain E. coli. It suggested that the drug-resistant plasmids of the Vibrio spp. could be transferred to E. coli, and the resistant plasmid had participated in the resistance mechanism for the resistance of the bacteria to aminoglycosides, such as neomycin and kanamycin. This study will provide basis data and an important reference for the study on the transmission for resistance to aminoglycoside between environmental vibrios and clinical bacteria. |
| Key words: pathogenic vibrio antibiotic-resistant plasmid neomycin kanamycin drug resistance mechanism |