| 摘要: |
| 采用水提醇沉法制备海蒿子多糖，利用红外光谱法（FT-IR）和PMP柱前衍生化法研究海蒿子多糖的理化特性及糖元组成，并采用实时荧光定量PCR法和ELISA法，研究免疫相关因子和细胞凋亡相关因子的mRNA表达。结果表明，海蒿子多糖总糖含量为24.32%，硫酸根含量为5.39%，具有α糖苷键。单糖分析表明，海蒿子多糖主要由半乳糖、岩藻糖、甘露糖组成。进一步体外免疫实验结果表明，海蒿子多糖具有促进小鼠巨噬细胞RAW264.7增殖的作用，能够提高巨噬细胞系免疫因子IL-1β、IL-6、iNOS和TNF-α的mRNA表达量。此外，海蒿子多糖还能抑制肺癌细胞A549的增殖，提高Bax/Bcl-2的比例，说明其具有抑制肿瘤的效果。ELISA分析表明，海蒿子粗多糖能显著提高RAW264.7分泌NO、IL-1β、TNF-α和IL-6细胞因子。因此，海蒿子多糖不仅具有免疫增强活性，还具有一定的抗肿瘤活性。 |
| 关键词: 海蒿子 多糖 结构组分 生物活性 |
| DOI： |
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| 基金项目:山东省自然基金(ZR2017BD019)；青岛科技大学2019年创新训练计划项目（201910426018） |
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| Structural Components and Biological Physicochemical Properties and Immunomodulatory Activities of Crude Polysaccharides from Sargassum pallidum |
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liyizhen1, tianxin2, Yu Zhiyang3, songlin2
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1.Qingdao Agricultural University;2.Qingdao University of Science and Technology;3.Qingdao Agriculture University
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| Abstract: |
| A natural polysaccharide SPPS was extracted from Sargassum pallidum by water extraction and alcohol precipitation method. We used infrared spectroscopy (FT-IR) and PMP pre-column derivatization to study the physical and chemical properties and monosaccharide composition of SPPS.The real-time fluorescence quantitative PCR method and ELISA method were used to study the mRNA expression of immune-related factors and apoptosis-related factors. The total sugar content of SPPS was 24.32%, and the sulfate content was 5.39%. FT-IR spectra analysis indicated that SPPS containsulfuric acid groups, and has α-glycosidic bonds. As for the monosaccharide composition,the most abundant componentsin SPPS were galactose, fucose and mannose. Bioassays indicated that SPPS could significantly enhance the proliferation of RAW264.7 macrophage ;and also stimulate the corresponding mRNA expression levels of iNOS and interleukins (IL-6 , IL-1β and TNF-α) in adose-dependent manner. In addition, SPPS could effectively suppress the proliferation of A549 cell line and increase the proportion of Bax/Bcl-2, indicating that it also has the effect of inhibiting the growth of A549cells. ELISA analysis showed that the crude SPPS can significantly increase the secretion of NO, IL-1β, TNF-α and IL-6 cytokines by RAW264.7. All these results indicated that The crude polysaccharide of Sargassum pallidum has not only immunomodulatory ability, but also has certain anti-tumor activity. |
| Key words: Sargassumpallidum polysaccharides structural componentss biological activity |