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琼枝卡拉胶与海萝胶结构的13C-NMR分析
纪明侯1, 顾柯楠1
中国科学院海洋研究所
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13C-NMR SPECTROSCOPIC ANALYSIS OF CARRAGEENAN FROM EUCHEUMA GELATINAE AND FUNORAN FROMGLOIOPELTIS FURCATA
Abstract:
The carrageenan extracted from Eucheuma gelatinae collected irom South China and funoran from Gloiopeltis furcata from North China were fractionated on EDAE-Sephadex A 50 column, and the structure of their main fractions was studied using 13C-NMR spectros-copy. The 13C-NMR spectrum of the main fraction of Eucheuma showed that β-carrageenan or desulfated K-carrageenan composed of(1→3)-β-D-ga]actosyl-(1→4)-3, 6-anhydro-μ-D-gala-ctose disaccharide repeat unit is the predominant component, and K-carrageenan the next one. The 13C-NMR spectra of the major fractions of Gloiopeltis eluted with 1.0M and 2.5 M NaCl indicated they are agar-type polysaccharode, 6-OSO3-agarose, consisting of (1→3) -6-OSO3-β-D-galactosyl-(1→4)-3, 6-α-L-galactose disaccharide repeat unit as a nearly single constituent in block in agar molecules.
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