| 摘要: |
| 初步探讨从中国毛虾(Acetes chinensis)经碱性及中性蛋白酶降解获得的多肽(AP、NP)的降压作用及相关机制。采用Cushman-Cheung方法测定酶解多肽体外对血管紧张素酶(ACE)活性的影响;采用两肾一夹型建立肾血管性高血压大鼠模型,分别长期预防给药及急性治疗给药;应用GY-6088型多道生理参数分析记录仪和张力传感器测定、记录血压和呼吸;采用均相竞争法测定大鼠血浆中血管紧张素Ⅱ(AngⅡ)的含量。结果表明,2种酶解多肽体外均能明显抑制ACE的活性,AP和NP的半数抑制浓度 |
| 关键词: 多肽 肾血管性高血压 大鼠 血管紧张素Ⅱ(AngⅡ) 降压作用 |
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| Study of antihypertensive effect of polypeptides from Acetes chinensis on renovasular antihypertensive rats |
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| Abstract: |
| To investigate the antihypertensive effects of polypeptides prepared from Acetes chinensis on renovascular hypertensive rats(RHR0 and related mechanisms, polypeptides were prepared fromAcetes chinensis with alkaline and neutral proteases, and the effects of these two polypeptides on the activity of ACE were measured by Cushman-Cheung assay in vitro. RHR models were made with two-kidney one clip assay, and antihypertensive effects were studied in RHR pretreated with polypeptides for long course or by acute venous injection. Arterial blood pressure, respiration and plasma angiorensin II(AngII) were measured respectively. the twopolypeptides markedly inhibited the activity of ACE in vitro with IC50 of AP(15.61 mg/L) and NP(16.95 mg/L). They remarkably reduced the arterial blood pressure in the two cases referred above. Plasma Ang II of RHR greatly decreased after pretreated with the polypeptides. Respiration of RHR had the same variation trend as blood pressure. Moreover, AP showed stronger antihypertensive and Ang II-reducing effects than that of NP. the effects of 200 μ g/g AP were similar to that of 14 μ g/g captopril. Polypeptides from Acetes chinensis showed strong antihypertensive effects in RHR. One possible mechanisms underlying the antihypertensive effects may be associated with its actions on decreasing the production of Ang II. |
| Key words: polypeptides renovascular hypertensive rats AngII antihypertensive effect |
