| 摘要: |
| 用单因素分析法研究6-苄基氨基嘌呤（6-BA）、萘乙酸（NAA）和赤霉素（GA）3种植物激素对螺旋藻生长和代谢产物含量的影响。结果显示，添加NAA（0.4 mg/L）和GA（1.0 mg/L）分别使钝顶螺旋藻（Spirulina platensis）和极大螺旋藻（Spirulina maximum）生物量达到最大值，与对照相比提高了49.1%和65.2%，比生长速率增加了66.7%和28.6%。同样，激素可以显著地影响螺旋藻的代谢产物含量。对于钝顶螺旋藻，GA（0.1 mg/L）使胞外总糖含量增加5倍；GA（0.3 mg/L）使胞内总糖含量提高5倍；6-BA（0.2 mg/L）使胞内蛋白含量提高27.8%；NAA（0.2 mg/L）使SOD酶比活力提高3.47倍。对于极大螺旋藻，GA（0.3 mg/L）使胞外总糖含量增加1.32倍，使胞内总糖含量提高2倍；NAA（1.6 mg/L）使胞内蛋白含量提高66.7%，6-BA（1.6 mg/L）使SOD酶比活力提高3.47倍。因此植物激素对螺旋藻生长和代谢产物合成有明显的促进作用，该结果为高密度规模化生产奠定基础。 |
| 关键词: 螺旋藻 植物激素 总糖 蛋白 SOD |
| DOI： |
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| 基金项目:天津市科技攻关计划重点项目（06YFBZNC04200）；天津自然科学基金重点项目（08JCZDJC16600） |
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| Effect on biomass and the content of active components of Spirulina sp. by three phytohormones |
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CHEN Ying,ZHAO Pei,WANG Xue-qing,PANG Guang-chang
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| Abstract: |
| The effects of three phytohormones, 6-Benzyl aminopurine (6-BA), Naphthalene acetic acid (NAA) and Gibberellic acid (GA), on the biomass and content of active components of Spirulina sp. have been researched through a single factor test. It was shown that：added NAA (0.4 mg/L) to Spirulina platensis and GA (1.0 mg/L) to S. maximum respectively made the biomass maximize 49.1%,65.2% and the specific growth rate maximize 66.7%,28.6%. The regulated reaction of hormone made the Spirulina sp. reach the largest throughput. Due to the metabolization of S. platensis, 0.1 mg/L GA can increase the content of its extracellular total sugar by 5 times, intracellular total suger augmented 5 times on GA (0.3 mg/L). Intracellular protein increased 27.8% on 6-BA(0.2 mg/L). NAA(0.2 mg/L) could increase the level of SOD of S. platensis by 3.47 times. To S. maximum, total in extracellular suger augmented 1.32 times and 2 times on GA(0.3 mg/L). NAA(1.6 mg/L) and 6-BA(1.6 mg/L) could respectively increase the intracellular protein and SOD by 66.7% and 3.47 times. It was concluded that phytohormones can promote the growth of Spirulina sp. This result can establish the rationale for large-scale high-density production. |
| Key words: Spirulina Phytohormone Total suger Protein SOD |
