| 摘要: |
| 在高密度养殖条件下，进行单因素随机设计动物试验。用5种饲料（蛋白质水平31%、35%、39%、43%、47%，以A～E组表示）分别投喂平均体质量6.2 g±0.2 g、平均养殖密度3.1 kg/m3的凡纳滨对虾（Litopenaeus vannamei），探寻蛋白质营养对虾生长、免疫、水质、抗胁迫的影响特征。结果表明：（1） 中高蛋白质水平具有显著促进对虾生长的效果，随着蛋白水平的提高，特定生长率先增加后降低，饲料系数正好相反，D组两指标最佳，分别为110.98 %和2.54；C、D、E组差 |
| 关键词: 凡纳滨对虾（Litopenaeus vannamei） 蛋白质水平 生长性能 免疫力 抗胁迫 水质因子 |
| DOI： |
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| 基金项目:国家863计划重点项目(2006AA100305) |
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| Effects of dietary protein levels on growth and immunity of Litopenaeus vannamei in high density culture system |
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| Abstract: |
| An experiment was conducted for 60 days to determine the relationship between the different dietary protein content and the growth and immunity of Litopenaeus vannamei in high density culture system. 450 white shrimps (average initial weight 6.2±0.2 g) were assigned randomly to five treatments with three replicates within each treatment and were fed diets with 5 different protein levels 31%, 35%, 39%, 43% and 47% respectively in average density 3.1 kg/m3. The results were as follows: (1) Shrimps fed 43% dietary protein had the maximal special growth rate and the minimal feed conversion, there was no significant difference when the protein level was above 39%. (2) The total hemocyte counts, T-AOC activity, POD activity, total protein content, and white protein content in haemolymph were increased at first and then decreased with the increase of protein content in the diet. Compared with 31% protein content, the above 5 indicators of 43% protein content which was the best group were enhanced from 16.8 % to 33.9%(P<0.05). The SOD activity and oxyhemoly content reached a peak in 47% and 39% protein content respectively. (3) The concentrations of total ammonia, nitrite in the feeding water were increased significantly with the increasing of dietary protein level. The best group under low salinity stress has 43% protein content and had a significant difference with the shrimps fed 31% protein content. (4) Based on the results of the special growth rate, immune indicators, water quality and anti-stress abilities, the optimum protein requirement in the diet for sub-adult white shrimps in high density culture is 39％～43％. |
| Key words: Litopenaeus vannamei protein levels growth performance immunity anti-stress ability |
