| 摘要: |
| 从患乳化病的养殖三疣梭子蟹（Portunus trituberculatus）体组织内分离出病原菌——溶藻弧菌（Vibrio alginolyticus)，通过肌肉注射的方式对三疣梭子蟹进行感染实验，对照组注射等量的生理盐水，在感染后0、24、48和72 h分别采集不同处理组三疣梭子蟹的血淋巴、肌肉和肝胰腺组织，测定溶藻弧菌对其各组织抗氧化相关酶活的影响。结果表明：注射生理盐水的对照组三疣梭子蟹体组织中抗氧化酶和MDA含量在不同的采样时间变化不显著（P>0.05） |
| 关键词: 三疣梭子蟹（Portunus trituberculatus） 溶藻弧菌（Vibrio alginolyticus） 抗氧化酶活 脂质过氧化物 |
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| 基金项目:国家863计划项目（2006AA10A406）；山东省博士后创新项目（200702012）；中国博士后科学基金项目（20080431220） |
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| Effect on antioxidant enzyme system of Portunus trituberculatus infected by Vibrio alginolyticus |
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| Abstract: |
| The present study examined the changes occurring in the antioxidant defence status in haemolymph, muscle hepatopancreas and tissue of Portunus trituberculatus infected by Vibrio alginolyticus. It is a kind of bacteria -V. alginolyticu that was isolated from tissue of diseased P. trituberculatus farmed under natural conditions. The healthy crabs were infected with V. alginolyticu by intramuscular injection of the isolated bacteria and 0.9% Nacl solution as a control respectively. Activities of superoxide dimutase (SOD), glutathione peroxidase (GSH-px) and levels of lipid peroxides (MDA) were measured at 0 h, 24 h, 48 h and 72 h following the infection. The results showed: The control crabs did not exhibit any significant variation in SOD, GSH-px and MDA activities in the tissues. However, V. alginolyticu infection induced a significant decrease of SOD activities in haemolymph, and muscle hepatopancreas of infected crabs compared with the control (P＜0.05). The GSH-px activities of the haemolymph and hepatopancreas were higher at 24 h after infection, whereas decreased significantly in the 1ast course of infection compared with the control (P＜0.05). The MDA levels of different tissues were increased significantly in infected crabs with the infection time (P<0.05). The results of this study indicated that the tissue antoxidant enzyme activities in P. trituberculatus infected by V. alginolyticu were decreased and were consistent with MDA level. The anto-xidant defence system of crab was destroyed, and the abilities of preventing disease and disease resistance declined. |
| Key words: Portunus trituberculatus Vibrio alginolyticus Antioxidant enzyme system Lipid peroxidation |
