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| 大黄鱼野生与养殖群体遗传结构的比较研究 |
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陈 锦1,2, 黎中宝1,2, 方 秀3, 雷光高1,2, 张桂玲1,2, 赵斌丽1,2, 王展林1,2
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1.集美大学水产学院;2.集美大学水产生物技术研究所;3.福建闽威水产实业有限公司
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| 摘要: |
| 采用垂直板型不连续聚丙烯酰胺凝胶电泳技术对福建野生与养殖大黄鱼(Pseudosciaena crocea)4个群体(湄洲野生群体、宁德野生群体、宁德养殖群体、连江养殖群体)的遗传结构进行了比较研究。结果表明, 4 个大黄鱼群体的平均每位点有效等位基因数为1.1250～1.1293, 多态位点百分数为2.50%～18.75%, 观察杂合度为0.1250, 期望杂合度为0.0636～0.0677。湄洲湾野生群体的遗传多样性高于养殖群体。4 个大黄鱼群体间的遗传分化很低, 4 个大黄鱼群体间遗传距离在0.0000～0.0001, 平均遗传距离为0.0005, 遗传分化系数(Fst = 0.0004)低, 基因流(Nm = 609.6667)大。 |
| 关键词: 大黄鱼(Pseudosciaena crocea) 等位酶 遗传多样性 遗传分化 遗传结构 |
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| 基金项目:福建省高等教育新世纪优秀人才支持计划资助项目(闽教科〔2006〕35 号); 集美大学创新团队基金资助项目(2007A001); 福建省海洋与渔业厅科技项目 |
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| The genetic structure of wild and cultivated populations of Pseudosciaena crocea |
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CHEN Jin,LI Zhong-bao,FANG Xiu,LEI Guang-gao,ZHANG Gui-ling,ZHAO Bin-li,WANG Zhan-lin
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| Abstract: |
| Genetic structures of wild and cultivated populations of Pseudosciaena crocea were investigated using the assay of vertical slab polyacrylamide gel electrophoresis. The results showed that the mean effective number of alleles per locus (Ae) ranged from 1.1250 to 1.1293, the percentage of polymorphic loci (P) ranged from 12.50 % to 18.75 %, the observed heterozygosity (Ho) was 0.1250, and the expected heterozygosities (He) ranged from 0.0636 to 0.0677. The genetic differentiation among P. crocea populations was low. Genetic distance a among populations ranged from 0.0000 to 0.0001, genetic differentiaion index (Fst) among populations was 0.0004, and gene flow washigh (Nm =609.6667). |
| Key words: Pseudosciaena crocea allozyme genetic diversity genetic differention genetic structure |