| 摘要: |
| 利用大型海藻细胞的全能性获得了蜈蚣藻(Grateloupia filicina)、多管藻(Polysiphonia urceolata)和孔石莼(Ulva pertusa Kjellm)的无性系材料, 在此基础上分别构建了绒球状微球体、网状微球体和簇生状微球体3 种微球体形式, 这种微球体形式适合于大型海藻的生物反应器高密度培养。簇生状的孔石莼微球体在10 L 藻类生物反应器中的培养密度达5.81 g/L 鲜质量, 最高生长速度达到0.36 g/L/d 鲜质量, 最大比生长速率为0.103/d。藻类生物反应器高密度培养海藻无性系可用于海藻育苗、水产养殖水处理以及天然活性物质生产等。 |
| 关键词: 海藻无性系 光生物反应器 微球体 高密度培养 |
| DOI： |
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| 基金项目:广东省高校水体富营养化与赤潮防治重点实验室开放基金资助项目(JN2010-2) |
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| Studies on developments of microspheres from macroalgal clones and cultivations in airlift photobioreactor |
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| Abstract: |
| Three macroalgal clones of Grateloupia filicina, Polysiphonia urceolata and Ulva pertusa, respectively, were obtained by the totipotency of marine algae. From them, three types of algal microspheres suitable for cultivation in a 10-L airlift photobioreactor were developed, which were flossy, netty and fascicular, respectively. The tufted microspheres of U. pertusa were cultivated in the photobioreactor to the biomass density of 5.81 g/L FW L, with the maximum growth rate of 0.36 g/(L?d)FW and specific growth rate of 0.103 d?1. The technology of high-density culture of macroalgae in photobioreactor will have wide applications, including seeding, mass cultivation, integrate-aquaculture, and manufacture of bioactive products. |
| Key words: macroalgal clone photobioreactor microsphere high-density cultivation |
