| 摘要: |
| 从处于不同脱水复水阶段的条斑紫菜(Porphyra yezoensis Ueda)叶状体中提取总RNA, 用荧光定量PCR技术检测条斑紫菜不同脱水和复水过程中psbA和psbD基因的转录水平上的相对表达量的变化。实验结果表明, psbA和psbD基因转录水平上的相对表达量的变化趋势相似但变化幅度不一致。在相对含水量约为71%时两种基因转录水平上的相对表达量分别增加了6.18 倍和2.13 倍。说明在条斑紫菜叶状体脱水过程中两种基因的转录并非同比例完成; 在轻度脱水状态下psbA和psbD基因转录水平上的相对表达量的快速增大可能是藻体暴露在空气中时适应陆地环境的初始响应。 |
| 关键词: 条斑紫菜(Porphyra yezoensis Ueda) 脱水复水 psbA psbD 转录水平上的相对表达量 |
| DOI： |
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| 基金项目:国家自然科学基金项目(30830015, B49082401); 科技部“十一五”国家科技支撑计划项目(2006BAD09A04); 国家863计划项目(2006AA10A402, 2007AA09Z406, 2006AA10A413) |
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| Effect of dehydration on the expressions of psbA and psbD genes of Porphyra yezoensis |
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| Abstract: |
| The transcription changes of psbA (encoding D1 protein) and psbD (encoding D2 protein) genes of Porphyra yezoensis during dehydration were determined with real-time PCR. The results showed that the relative quantification of transcript levels of psbA and psbD genes generally appeared to vary in a similar manner but their variation ranges differed to some extent. The moderate dehydration of about 71% RWC (relative water content) increases the relative transcript levels for psbA and psbD by about 6.18 and 2.13-fold, respectively, suggesting that D1 protein and D2 protein might be differentially synthesized. The significant increases of the relative quantification of transcript levels may be the initial response of P. yezoensis to the air to adjust to the terrestrial environment. |
| Key words: Porphyra yezoensis, dehydration and rehydration, psbA, psbD, the relative quantification of transcript level |
