| 摘要: |
| 采用改进的Bligh-Dyer 法提取脂溶性成分, 气相色谱-质谱联用法(GC-MS)进行分离和鉴定,C19:0 内标确定总脂及各组分含量, 研究了鼠尾藻(Sargassum thunbergii)、浒苔(Enteromorpha prolifera)、龙须菜(Gracilaria lemaneiformis)和红毛菜(Bangia sp.)4 种经济海藻的脂肪酸组成及含量。结果表明, 4 种海藻都检测出C14-C22 脂肪酸, 总脂含量在12～19 mg/g 之间, 不饱和脂肪酸为主要组成成分,含量均超过60%。不饱和脂肪酸中以多不饱和脂肪酸(PUFAs)为主, 富含n-3 和n-6 系列PUFAs, n-6 与n-3 系列PUFAs 之比均低于2。比较4 种海藻脂肪酸组成特点表明, 鼠尾藻以C16、C18 和C20 为主要组成成分, 具褐藻类脂肪酸组成特征; 浒苔以C16 和C18 为主要组成成分, 具绿藻类脂肪酸组成特征;龙须菜和红毛菜以C16 和C20 类脂肪酸为主, 具典型红藻类脂肪酸组成特征, 同时二者又有不同之处,分别显示真红藻与原始红藻脂肪酸组成的特点。 |
| 关键词: 鼠尾藻(Sargassum thunbergii) 浒苔(Enteromorpha prolifera) 龙须菜(Gracilaria lemaneiformis) 红毛菜(Bangia sp.) 脂肪酸 气相色谱质谱法 |
| DOI： |
| 分类号: |
| 基金项目:国家863 计划项目(2006AA10A413)资助 |
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| Analysis of the fatty acid composition of four economic seaweeds |
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| Abstract: |
| The fat-soluble components were extracted using a modified Bligh-Dyer method, which were further separated and identified by gas chromatography-mass spectrometry (GC-MS), with C19:0 as internal control. The fatty acid composition of four economic algaes including Sargassum thunbergii, Enteromorpha prolifera, Gracilaria lemaneiformis and Bangia sp. were determined. The C14-C22 fatty acids were detectable in all of the four algae with total lipid contents varying between 12-19 mg/g. The unsaturated fatty acids were the main components among the fatty acids, which accounted for more than 60%. The polyunsaturated fatty acids (PUFAs), especially the n-6 and n-3 PUFAs, were the main components among the unsaturated fatty acids. The content ratios of n-6/n-3 PUFAs were all less than 2. The comparison of fatty acid composition showed that fatty acid designated C16, C18 and C20 were the major composition in S. thunbergii, exhibiting typical fatty acid composition characteristic of brown algae. C16 and C18 were the major fatty acid composition in E. prolifera, the typical characteristic of green algae. Both G. lemaneiformis and Bangia sp. mainly contained C16 and C20, which was the typical characteristic of red algae. Meanwhile, G. lemaneiformis and Bangia sp. exhibited typical composition characteristic of Florideophycidae and Bangioideae, respectively. |
| Key words: Sargassum thunbergii Enteromorpha prolifera Gracilaria lemaneiformis Bangia sp. fatty acid GC-MS |
