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一株桑沟湾赤潮藻的分子鉴定
徐 娜1,2, 逄少军1, 刘 峰1
1.中国科学院 海洋研究所;2.中国科学院 研究生院
摘要:
2011 年5 月山东荣成桑沟湾近海海域暴发赤潮, 其规模在桑沟湾历史上罕见, 较严重地影响了该海域水产动物的人工养殖。本文作者分离得到了赤潮暴发过程中海水里的一株优势种, 并对其核糖体18S RNA 基因(18S rDNA)和转录单元内间隔区(ITS)进行了序列扩增和分析。依据这两种序列构建的系统进化树都显示这株藻与卡罗藻Karlodinium veneficum(=K. micrum)完全聚在一起, 因此推断出其应为K. veneficum, 是一种常见有害赤潮藻。本文是卡罗藻在桑沟湾海域暴发的首次报道。
关键词:  桑沟湾  赤潮  卡罗藻  分子鉴定
DOI:
分类号:
基金项目:国家科技基础条件平台——水产种质资源平台运行项目(2006DKA30470_017); 中国科学院野生生物种质库海藻种质库平台运行项目
Molecular identification of a bloom-forming species isolated from Sanggou Bay in Shandong Province
Abstract:
A seldom seen red tide occurred in Sanggou Bay, Shandong province, China in May, 2011 and the local shellfish aquaculture was greatly affected. During this event, we have isolated a dominant alga strain and we augmented and analyzed its 18S ribosomal DNA and internal transcribed spacer sequences. The phylogenetic trees based on the two sequences both revealed that this species clusters with Karlodinium veneficum(=K. micrum). So it is identified as K. veneficum, one of harmful algal bloom species reported in the Southern Sea of China previously. This is the first report of this alga to appear in bloom in Sanggou bay.
Key words:  Sanggou Bay  red tide  Karlodinium  molecular identification
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