| 摘要: |
| 对采自海南东寨港的海洋红树林植物黄瑾(Hibiscus tiliaceus)叶中分离到的一株内生真菌Aspergillus sydowii EN-198 的次生代谢产物进行了研究。利用正相与反相硅胶柱层析、葡聚糖凝胶Sephadex LH-20 柱层析以及制备薄层层析(PTLC)等分离手段从其发酵液中分离得到14 个化合物, 通过一维、二维核磁共振技术、质谱技术等鉴定了所有化合物的结构, 分别为: 环-(S-脯氨酸-S-苯丙氨酸)(1), 环-(S-脯氨酸-S-亮氨酸)(2), 环-(S-苯丙氨酸-S-色氨酸)(3), (1S)-1-(4′-间羟基苯甲酸)-1,1,5,5-二甲基己二醇(4), 曲酸(5), N-[2-(4-吲哚)乙基]乙酰胺(6), N-[2-(4-对羟基苯酚)乙基]乙酰胺(7), 胸腺嘧啶脱氧核苷(8), 尿嘧啶脱氧核苷(9), 尿嘧啶核苷(10), 过氧化麦角甾醇(11), 麦角甾醇(12),(2S,2′R,3R,3′E,4E,8E)-N-(2′-羟基-3′-十六烯酰基)-9-甲基-4,8-二十碳二烯-1,3-二醇(13)以及1-O-β-D-葡萄糖基-(2S,2′R,3R,3′E,4E,8E)-N-(2′-羟基-3′-十六烯酰基)-9-甲基-4,8-二十碳二烯-1,3-二醇(14); 其中化合物1 和2 为首次从Aspergillus sydowii 中分离得到。对所有化合物测试其抑菌活性, 发现化合物5 与7 对金黄色葡萄球菌有较好抑制活性。 |
| 关键词: 红树林 黄槿 内生真菌 化学成分 结构鉴定 |
| DOI： |
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| 基金项目:国家自然科学基金(30910103914)和科技部“973计划”项目(2010CB833802) |
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| Chemical constituents of Aspergillus sydowii EN-198, an endophytic fungus derived from the marine-mangrove plant Hibiscus tiliaceus |
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| Abstract: |
| Cultivation of the fungal strain Aspergillus sydowii EN-198, an endophytic fungus which was isolated from the healthy fresh leaf of marine-mangrove plant Hibiscus tiliaceus, resulted in the identification of fourteen compounds. These compounds were isolated by a combination of silica gel, Sephadex LH-20, and Lobar LiChroprep RP-18 column chromatography as well as the preparative thin layer chromatography (PTLC). The structures were elucidated by analysis of their spectroscopic data including MS, 1D and 2D NMR to be L, L-cyclo(phenylalanyl-prolyl) (1), L, L-cyclo(leucyl-prolyl) (2), L, L-cyclo(phenylalanyl-tryptophyl) (3), Hydroxysydonic acid (4), Kojic acid (5), N-Acetyltryptamine (6), N-[2-(4-Hydroxyphenyl)ethyl]acetamide (7), Thymidine (8), 2′-Deoxyuridine (9), Uridine (10), (22E,24R)-5α,8α-epidioxyergosta-6,22-dien-3β-ol (11), (22E,24R)-ergosta-5,7,22-trien-3β-ol (12), Asperamide A (13) and B (14). Compounds 1 and 2 were firstly reported to be isolated from Aspergillus sydowii, while 5 and 7 exhibited moderate antibacterial activity against S. aureus. |
| Key words: Hibiscus tiliaceus Endophytic fungus Aspergillus sydowii EN-198 chemical constituents structure elucidation |
