| 摘要: |
| 采用2216E、R2A、M1 培养基, 首次从俄罗斯库页岛潮间带沉积物样品中分离到82 株可培养海洋细菌, 经查重并菌后选取其中45 株具有代表性的菌株进行了16S rRNA 基因序列分析和多样性研究。结果发现, 它们共分布于变形细菌门(Proteobacteria)、放线菌门(Actinobacteria )、拟杆菌门(Bacteroidetes)和厚壁菌门(Firmicutes)4 个类群(分别占45 株代表菌株的42.2%、20.0%、20.0% 和17.8%), 代表着6 个纲、27 个属、44 个种; 同时发现其中有8 个潜在新种和1 个潜在新属。本研究结果表明, 俄罗斯库页岛潮间带沉积物可培养细菌的系统发育多样性非常高, 大部分菌株与分离自日本和韩国近海的细菌物种在系统发育关系上高度同源, 但也存在不少潜在的新物种。 |
| 关键词: 库页岛 潮间带 细菌 多样性 |
| DOI：10.11759/hykx20141109001 |
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| 基金项目:国家青年自然科学基金资助项目(31200005); 中国科学院海洋研究所“一三五”专项资助(2012IO060103) |
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| Phylogeny diversity of culturable bacteria from intertidal sediments samples of Russian Sakhalin Island |
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| Abstract: |
| Four intertidal sediment samples collected from Russian Sakhalin Island were treated with plate dilution method and the culturable marine bacteria were isolated. The diversity of marine bacteria was examined based on 16S rRNA gene sequence analysis. A total of 45 representative strains were selected from 82 isolates, and were further sequenced and the phylogenetic analysis was carried out. The results showed that the 45 representative strains were composed of 4 major lineages including Proteobacteria (42.2%), Actinobacteria (20%), Bacteroidetes (20%) and Firmicutes (17.8%), and belonged to 6 classes, 27 genera and 44 species. Meanwhile, 8 strains were identified as potential novel species and 1 strain was identified as potential novel genus. This study indicated that there was high bacterial diversity in intertidal sediment samples of Russian Sakhalin Island and most of the strains were very close to the bacteria species isolated from coastal area of Japan and South Korea. In addition, many potential new species were found. |
| Key words: Sakhalin Island intertidal sediment marine bacteria diversity |
