| 摘要: |
| 硅藻是一种在全世界水环境中都有发现的单细胞真核藻类，其特有的硅质壁使其成为海洋生物硅的最大贡献者，它们在全球碳/硅元素的生物地球化学循环中扮演至关重要的角色。亲硅蛋白（silaffins）是一种分离于硅藻硅质壁，并与其硅质化密切相关的蛋白。亲硅蛋白可以在体外硅酸溶液中沉淀二氧化硅（SiO2），这种活性来自其特有的蛋白序列和翻译后修饰调节。基于对亲硅蛋白结构的理解，多种与其相似的仿生多肽被应用到人工SiO2的合成中。本文综述了硅藻亲硅蛋白的分离鉴定、功能以及其在生物技术和生物医药方面的应用，为亲硅蛋白基础研究和应用潜力提供基础。 |
| 关键词: 亲硅蛋白 蛋白分离 翻译后修饰 仿生SiO2 |
| DOI：10.11759/hykx20181224002 |
| 分类号:Q71 |
| 基金项目:国家自然科学基金（41876134，41676112，41276124）；天津市高等学校创新团队培养计划（TD12-5003）；天津市131创新团队（20180314）；教育部长江学者奖励计划 |
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| State of the art of the diatom silica-forming protein silaffins |
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XIN Ye-hong1,2, SHANG Xiao-mei1,2, SUN Jun1,2
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1.Tianjin Key Laboratory of Marine Resources and Chemistry, Tianjin University of Science and Technology, Tianjin 300457, China;2.Research Centre for Indian Ocean Ecosystem, Tianjin University of Science and Technology, Tianjin 300457, China
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| Abstract: |
| Diatoms are single-celled eukaryotic microalga and can be found in all aquatic environments around the world. Their unique siliceous cell wall makes them the largest contributor to marine biogenic silica. Diatoms play a vital role in the global biogeochemical cycles such as the carbon cycle and the silicon cycle. Silaffins are proteins that are closely related to the silicification of diatom cell walls. Silaffins can precipitate silica from silicic acid solution in vitro. This type of activity is derived from its unique protein sequence and posttranslational modification. Based on the knowledge of the structure of silaffins, a variety of biomimetic polypeptides similar to silaffins have been applied in the synthesis of artificial SiO2. This paper reviews the isolation, identification, function, and the biotechnological and biomedical applications of silaffins. This review article is intended to contribute to the basic research and practical application of silaffins. |
| Key words: silaffins separation of proteins posttranslational modification Biomimetic Silica |
