| 摘要: |
| 为研究异养培养的蛋白核小球藻(Chlorella pyrenoidosa)去除对虾池塘养殖尾水氮、磷营养盐的效果, 本实验通过对异养藻种的光自养转换、盐度驯化, 并以不同初始密度接种入养殖尾水, 定期检测水体中氨氮、磷酸盐、硝酸盐氮和亚硝酸盐氮等指标, 分析其对营养盐的吸收利用规律。结果表明: 光照强度6 600 lx、光照周期16 h∶8 h(L/D)、温度25±0.5℃条件下, 接种细胞密度为1.0×105个/mL、5.0×105个/mL、1.0×106个/mL的蛋白核小球藻均在接种初期快速进入指数生长期, 10 d后进入生长平台期, 20 d藻细胞生物量分别增加39.25、7.98和4.07倍; 蛋白核小球藻能明显降低养殖水体中氮、磷营养盐浓度, 起到去氮除磷的净化作用, 磷酸盐的去除率随藻细胞的生长持续上升, 21 d去除率分别为58.8%±0.72%、72.9%±1.7%、81.4%±9.86%; 对氮盐的利用规律依次为氨氮、硝酸氮和亚硝酸氮, 氨氮6 d的去除率达81.9%±6.0%, 96.2%±1.16%, 95.4%±1.24%, 硝酸氮21 d去除率达82%±1.35%、93.3%±4.41%、91.8%±2.77%, 亚硝酸氮21 d去除率分别为84.3%±3.52%、23.5%±2.53%、3.4%±2.16%。相关结果可为异养蛋白核小球藻在海水池塘养殖尾水水质净化的应用提供参考。 |
| 关键词: 蛋白核小球藻 异养培养 养殖尾水 水质净化 |
| DOI：10.11759/hykx20220507003 |
| 分类号:S949 |
| 基金项目:中国科学院海洋大科学研究中心重点部署项目(COMS2019J07);山东省重点研发计划(科技示范工程)课题资助(2021SFGC0701) |
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| Experiment on purification of tail water of shrimp pond culture with heterotrophic cultivated Chlorella pyrenoidosa |
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SONG Chu-er1,2, MENG Zhen2, ZHANG Zheng2, SONG Xiao-jin3, GAO Chun-ren2
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1.School of Fishery, Zhejiang Ocean University, Zhoushan 316022, China;2.Laboratory for Marine Fisheries Science and Food Production Processes, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China;3.Qingdao Institute of Bioenergy and Processes, Chinese Academy of Sciences, Qingdao 266101, China
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| Abstract: |
| To investigate the removal ability of inorganic N and P nutrients in the tail water of Pacific white shrimp (Penaeus vannameri) pond culture by heterotrophic cultivated Chlorella pyrenoidosa, various initial densities of the microalgae were inoculated into the tail water. The indexes of phosphate, ammonia nitrogen, nitrate nitrogen, and nitrite nitrogen in the tail water were detected regularly to analyze the absorption and utilization of nutrients. The results demonstrated that under the conditions of light intensity of 6 600 lx, light–dark cycle of 16︰8, and temperature of 25℃±0.5℃, the growth rate of Chlorella pyrenoidosa with three inoculation densities (1.0×105cell/mL, 5.0×105cell/mL, and 1.0×106cell/mL, respectively) proliferated within first 10 days, thereafter gradually diminishing. On the 20th day, the biomass of algae cells multiplied by 39.25, 7.98, and 4.07 times, respectively. Chlorella pyrenoidosa has the potential to significantly reduce the concentration of nitrogen and phosphate in the tail water. The phosphate removal rate increased with the growth of algae cells, and the removal rates were 58.8%±0.72%, 72.9%±1.7%, and 81.4%±9.86% on the 21st day, respectively. The utilization of nitrogen salts was followed by ammonia nitrogen, nitric nitrogen, and nitrite nitrogen. The removal rates were 81.9%±6.0%, 96.2%±1.16%, 95.4%±1.24% for ammonia nitrogen on the 6th day, 82%±1.35%, 93.3%±4.41%, 91.8%±2.77% for nitric nitrogen on the 21st day, and 84.3%±3.52%, 23.5%±2.53%, 3.4%±2.16% for nitrite nitrogen on the 21st day, respectively. The results provide a reference for the application of heterotrophic cultivated Chlorella pyrenoidosa in the purification of the tail water in marine pond culture. |
| Key words: Chlorella pyrenoidosa heterotrophic cultivation aquaculture tail water water purification |
