首页 | 期刊介绍 | 编委会 | 征稿启事 | 投稿指南 | 常用下载 | 过刊浏览
引用本文:
【打印本页】   【HTML】   【下载PDF全文】   查看/发表评论  【EndNote】   【RefMan】   【BibTex】
←前一篇|后一篇→ 过刊浏览    高级检索
本文已被:浏览 191次   下载 380 本文二维码信息
码上扫一扫!
分享到: 微信 更多
具产电性能光合细菌DH-3的分离及其对多种底物污水处理能力的评价
于士翔1,2, 王文静1, 刘峰2, 刘芳华1
1.中国科学院烟台海岸带研究所 海岸带生物学与生物资源利用重点实验室, 烟台 264003;2.中国农业大学烟台研究院 海洋学院水产养殖学系, 烟台 264670
摘要:
本文通过传统微生物分离培养方法从富营养化环境中分离培养出可高效处理多种底物的污水并具备胞外电子传递能力和产电潜力的兼性厌氧光合细菌。在光照培养条件下,利用改良的能够将光合细菌富集/分离的培养基分离培养光合细菌,从形态学和16S rDNA的分子生物学角度鉴定菌株。运用多个指标检测光合细菌处理不同底物的污水培养基的能力,如化学需氧量(chemical oxygen demand,COD)、总氮(total nitrogen,TN)、总磷(total phosphate,TP),并在电化学工作站检测光合细菌的产电能力。结果表明,获取的光合细菌菌株DH-3与光合细菌类球红细菌(Rhodobacter sphaeroides)有97%的相似性。该菌株拥有在多种底物的污水培养基中生长的能力,其中在以乙酸为底物的培养基中生长最好。通过检测发现该菌株对污水的COD、TN和TP均具有较高的去除率。电化学工作站检测结果表明,菌株DH-3的最大产电流量能达到7.5mA/m2,最大功率密度达到0.056W/m2。由此可见,光合细菌DH-3在污水处理及资源再循环利用等方面具有十分重要的应用潜力。
关键词:  光合细菌  产电性能  化学需氧量  污水处理  有机物降解
DOI:10.12036/hykxjk20170705001
分类号:X703
基金项目:国家自然科学基金青年基金(31600370,41401285);国家自然科学基金面上项目(41371257,41573071);中国科学院“百人计划”。
Isolation and Application Potential of Photosynthetic Bacterium Strain DH-3 with Electricity Production in the Wastewater Treatment
YU Shi-Xiang1,2, WANG Wen-Jing1, LIU Feng2, LIU Fang-Hua1
1.Key Laboratory of Coastal Biology and Biological Resources Utilization, Yantai Institute of Coastal Zone Research, Chinese Academy of Sciences, Yantai 264003, China;2.China Agriculture University, School of Ocean, Yantai 264670, China
Abstract:
In this study, from a eutrophic lake we enriched and separated photosynthetic bacteria, which can consume sewage as well as initiate extracellular electron transfer and thereby produce electricity. We isolated and purified photosynthetic bacteria from improved enriched media and isolated media and identified them using morphological and molecular methods. We used the national standard method and spectrophotometry to determine the chemical oxygen demand, total nitrogen, and total phosphate. To detect the electricity production of the photosynthetic bacteria, we used an electrochemical workstation. Our results show that the photosynthetic bacteria strain DH-3 has high similarity with Rhodobacter sphaeroides. We found the removal rates of COD, TN, and TP to decrease in the wastewater medium. The electrochemical workstation detected that strain DH-3 produced maximum electricity at 7.5 mA/m2 and maximum power at 0.056 W/m2. Our results indicate that strain DH-3 has potential application in future wastewater treatment and resource recovery technologies.
Key words:  Photosynthetic bacteria  Electricity production  Chemical Oxygen Demand  Sewage treatment  Organic matter degradation
版权所有 《海洋科学集刊》编辑部 Copyright©2008 All Rights Reserved
主管单位:中国科学院 主办单位:中国科学院海洋研究所
地址:青岛市南海路七号  邮编:266071  电话:0532-82898769  E-mail:luoxuan@qdio.ac.cn
技术支持:北京勤云科技发展有限公司
欢迎关注微信公众号