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引用本文:赵玉超,秦浩,李娜,沈敏,王仁杰,李玉全.神经递质多巴胺(DA)和5-羟色胺(5-HT)对三种主要养殖对虾争胜行为的影响.海洋与湖沼,2017,48(3):628-633.
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神经递质多巴胺(DA)和5-羟色胺(5-HT)对三种主要养殖对虾争胜行为的影响
赵玉超, 秦浩, 李娜, 沈敏, 王仁杰, 李玉全
青岛农业大学海洋科学与工程学院 青岛 266109
摘要:
本文采用肌肉注射和行为分析的方法,进行了注射多巴胺(DA)和5-羟色胺(5-HT)对对虾争胜行为的研究,比较分析了注射多巴胺(DA)和5-羟色胺(5-HT)对日本囊对虾(Penaeus japonicus)、凡纳滨对虾(Litopenaeus vannamei)和中国明对虾(Fenneropenaeus chinensis)争胜行为的影响。多巴胺注射浓度为2×10-3mol/L、2×10-4mol/L,5-羟色胺注射浓度为2×10-5mol/L、2×10-6mol/L,以生理盐水为对照,各设3个平行组。检测注射1h后对虾体内的DA、5-HT含量,分析比较注射DA、5-HT前后及不同对虾种类争胜行为的差异。结果表明,注射多巴胺后三种对虾间的争斗显著增加,HDA比LDA更易激发对虾间的争斗,同种对虾在注射不同浓度多巴胺后与对照组差异均显著(P<0.05);相较于对照组,南美白对虾、日本囊对虾、中国明对虾在注射低浓度多巴胺后,对虾平均争斗次数分别增加13.8%、11.1%、21.8%,高浓度多巴胺组分别增加91.0%、64.0%、68.5%;对虾平均争斗次数随着DA浓度的升高而增加;平均优势指数总体呈下降趋势。随着5-HT浓度的增加,对虾的平均争斗次数随之减少,相较于对照组,低浓度5-羟色胺组降幅超过50%,高浓度5-羟色胺组降幅超过270%以上。争斗次数表现为中国明对虾 > 凡纳滨对虾 > 日本囊对虾。平均优势指数随注射5-HT浓度的增加而小幅度上升。
关键词:  对虾  多巴胺  5-羟色胺  争胜行为
DOI:10.11693/hyhz20161200293
分类号:S967;Q958.12
基金项目:国家自然科学基金项目,31101916号;山东省现代农业产业技术体系虾蟹类创新团队,SDAIT-15-011号;青岛市科技成果转化引导计划,14-2-4-87-jch号。
附件
EFFECTS OF NEUROTRANSMITTER DOPAMINE AND 5-HYDROXYTRY PTAMINE ON AGONISTIC BEHAVIOR OF THREE FARMED SHRIMP
ZHAO Yu-Chao, QIN Hao, LI Na, SHEN Min, WANG Ren-Jie, LI Yu-Quan
College of Marine Science and Engineering, Qingdao Agricultural University, Qingdao 266109, China
Abstract:
Effects of injecting biogenic amines (dopamine, 5-hydroxytry ptamine) on agonistic behavior of Penaeus japonicus, Litopenaeus vannamei, Fenneropenaeus chinensis were studied. Injectable concentration of dopamine (DA) and 5-hydroxytry ptamine (5-HT) were 2×10-3mol/L, 2×10-4mol/L and 2×10-5mol/L, 2×10-6mol/L, respectively. Saline was used as control, three replicates. The content of DA and 5-HT in shrimp were detected an hour after injection, the differences of agonistic behavior of shrimp were analyzed and compared before and after injection of DA and 5-HT. The results show that, after injecting dopamine, the fight of three kinds of shrimp significantly increased, HDA is more likely to stimulate the struggle than LDA in shrimp, the difference was significant in the same group (P<0.05). Compared with the control group, Litopenaeus vannamei, Penaeus japonicus and Fenneropenaeus chinensis, the average number of shrimp were increased by 13.8%, 11.1% and 21.8% after injecting of low concentration dopamine, the high concentrations of dopamine group were increased by 91%, 64% and 68.5%; the average number of fighting between shrimps increased with increasing DA concentration, significantly higher than the saline group, the average superiority index descended generally. 5-HT can effectively reduce the fight between the same species of shrimp, the number of fights between each treatment group was significant (P<0.05). With the increase of 5-HT concentration, the average number of fighting in shrimp was gradually reduced. Compared with the control group, the low concentration of 5-HT group is down more than 50%, the high concentration of 5-HT group is down more than 270%. The number of fighting shows that P. japonicus > F. chinensis > L. vannamei, the average superiority index increased with the increase of 5-HT concentration.
Key words:  shrimp  DA  5-HT  agonistic behavior
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