| 摘要: |
| 对分布在黄、渤海沿岸的单条胶黏藻Dumontia simplex Cotton的形态结构、生物量、成熟个体比例与成熟个体生物量/总生物量（R/T指数）的变化、温度性质以及rbcL、COI基因序列进行了详细研究。结果表明：（1）10个采集地点的单条胶黏藻藻体直立、扁平或螺旋状，质地胶质黏滑，颜色为红色或紫红色。在大连黑石礁与付家庄采集到的藻体长度和宽度明显高于其他采集地点；（2）藻体由皮层与髓部组成，皮层由4—8层细胞组成，髓部有疏松的根丝细胞。囊果散生，包埋于皮层内，刚发育的囊果较小，色淡，随着藻体生长囊果变大，颜色加深。四分孢子囊侧生在内皮层上，成熟的四分孢子囊呈十字形分裂；（3）4月生物量最大，平均生物量为4.384g/m2。12月至次年6月成熟个体比例逐渐增大，6月达到100%，且均为四分孢子体；（4）单条胶黏藻生长周期为12月至次年6月，属于一年生海藻，温度性质属于亚寒带性；（5）rbcL与COI基因序列分析表明，十个采集地点的样本间无碱基差异，与产自美国单条胶黏藻聚在一起，形成一个独立的分支，均确定为单条胶黏藻。 |
| 关键词: 单条胶黏藻 形态结构 生物量 温度性质 分子分析 |
| DOI：10.11693/hyhz20171200307 |
| 分类号:Q949.29 |
| 基金项目:国家自然科学基金项目，31270251号。 |
附件 |
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| GROWTH STATUS AND MOLECULAR SYSTEMATICS OF DUMONTIA SIMPLEX |
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WANG Yong-Yu, WANG Hao-Lin, LI Jia-Jun, WANG Hong-Wei
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College of Life Sciences, Liaoning Normal University, Dalian 116081, China
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| Abstract: |
| Dumontia simplex Cotton populations were sampled at 10 sites in the coast of the Yellow Sea and Bohai Sea in Shandong, Hebei, and Liaoning provinces, and their morphological structure, biomass variation, mature individual proportion, and temperature property, as well as the large subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase (rbcL) gene, and the partial cytochrome coxidase I (COI) gene sequences were analyzed. The results indicated that:(1) thallus of the 10 sites were erect and flattened or in heliciform, gelatinous in texture, red or purplish red, but the length and width of thallus in Heishijiao and Fujiazhuang sites in Dalian were significantly greater than the other samples. (2) Thallus were composed of cortex and medulla, the cortex had 4-8 layers, and the medulla was consisted of many loosened rhizoidal filaments. Cystocarps distributed and embedded throughout the whole thallus. The newly developed cystocarp was smaller and light-colored. With thallus growth, cystocarps gradually enlarged and became darker in color. The mature tetrasporangia were formed from the inner cortex cells, and cruciately divided. (3) The average maximum biomass was 4.384g/m2 appeared in April. From December to June, the proportion of mature individuals increased gradually, reaching 100% in June, and all of thallus were tetrasporophytes. (4) D. simplex belonged to annual algae. The growth period was from December to June. The temperature property of D. simplex is subfrigid. (5) According to the phylogenetic tree constructed by rbcL gene sequences and COI gene sequences, there were no sequence divergence among the 10 sites in this research. The 10 samples and that from USA clustered in a single monophyletic subclade; they were all identified as D. simplex. |
| Key words: Dumontia simplex morphological structure biomass temperature property molecular systematics |
