| 摘要: |
| 本研究通过设置温度突变组（15℃、25℃、31℃）和盐度突变组（5、15、25、35），探讨了温度、盐度变化胁迫对海洋青鳉鱼（Oryzias melastigma）摄食行为及抗氧化生理的影响。结果表明，在温度为15℃和31℃（盐度为25）时以及盐度为5和35（温度为25℃）条件下，青鳉鱼摄食响应时间增长，摄食量、摄食效率和摄食成功率显著下降（P<0.05）。超氧化物歧化酶（SOD）、过氧化氢酶（CAT）酶活性和丙二醛（MDA）含量在15—31℃时随温度增加先降低后增加。SOD酶活性31℃时第7天显著性低于对照组（P<0.05）。MDA含量在15℃和31℃时在显著性高于对照组，第7天显著性升高（P<0.05）。不同盐度处理组，SOD、CAT活性和MDA含量均随盐度增加先降低后增加，MDA含量在盐度为5和35时均显著高于对照组（P<0.05）。综上，海洋青鳉鱼在盐度15—25，水温25℃条件下摄食及游动行为活跃，抗氧化活性最低，生长良好。当温度高于31℃或低于15℃；盐度高于35或低于5时胁迫对青鳉鱼摄食行为及抗氧化性造成明显影响。 |
| 关键词: 温度胁迫 盐度胁迫 海洋青鳉鱼 摄食行为 抗氧化性 |
| DOI：10.11693/hyhz20181000234 |
| 分类号:Q955;S965 |
| 基金项目:教育部重点科技项目，212070号；宁波大学学科开放基金项目，xkzsc1508号；宁波市自然科学基金项目，2017A610282号。 |
附件 |
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| EFFECTS OF SHORT-TERM TEMPERATURE OR SALINITY STRESS ON FEEDING BEHAVIOR AND ANTIOXIDANT OF MARINE MEDAKA (ORYZIAS MELASTIGMA) |
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WANG Run-Ping, DAI Ling-Ling, CHEN Ya-Fei, XU Yong-Jian
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School of Marine Sciences/Key Lab of Applied Marine Biotechnology, Ministry of Education, Ningbo University, Ningbo 315211, China
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| Abstract: |
| The effects of variable temperature acute change (15℃, 25℃, 31℃) and salinity acute change (5, 15, 25, 35) on the feeding behavior and antioxidant of marine medaka (Oryzias melastigma) were investigated. The results show that the feeding response time increased, while the feed intake, feeding efficiency, and feeding success rate decreased significantly (P<0.05) at a temperature of 15℃ and 31℃. The SOD, CAT activities, and MDA content increased first and then decreased with the temperature increased from 15℃ to 30℃. The SOD activities increased significantly at 31℃ group on the 7th day (P<0.05). The MDA content significantly higher than the control group and increased significantly (P<0.05) at the 7th day at the temperature of 15℃ and 31℃. Also, SOD, CAT activities, and MDA content decreased at first and then increased with increased salinity from 5 to 35, and the content of MDA was significantly higher (P<0.05) than that of the control. Therefore, we believe that a temperature or salinity acute change can affect significantly the feeding behavior and antioxidant system of O. melastigma, and the suitable temperature was 25℃, salinity was 15-25. |
| Key words: Oryzias melastigma temperature stress salinity stress feeding activity antioxidation |
