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| 基于脂肪酸生物标记法探讨中国沿海六群体绒螯蟹(Eriocheir sensu stricto)的种群遗传 |
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张成1,2, 祖露1,2, 李清清1,2, 成永旭1,2,3, 吴旭干1,2,3
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1.上海海洋大学 农业部淡水种质资源重点实验室 上海 201306;2.上海海洋大学 水产动物遗传育种上海市协同创新中心 上海 201306;3.上海海洋大学 水产科学国家级实验教学示范中心 上海 201306
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| 摘要: |
| 水产动物肌肉中的脂肪酸组成可用于评价其种群遗传。本研究通过测定和比较辽河（LH）、黄河（HH）、长江（YZ）、瓯江（OJ）、闽江（MJ）和南流江（NLJ）水系野生绒螯蟹（Eriocheir sensu stricto）肌肉中的脂肪酸组成，进一步分析了6群体间的遗传多样性和亲缘关系。结果显示：（1）在6水系群体中检测出26种脂肪酸，其中C20：5n3、C18：1n9、C22：6n3、C16：0、C20：4n6、C18：2n6和C18：0为主要种类，其总和占总脂肪酸的74%以上；（2）逐步判别分析法筛选出8种判别贡献较大的脂肪酸，分别是C17：0、C18：0、C20：0、C22：1n9、C18：3n3、C18：3n6、C20：2n6和C22：5n3，据此分别建立判别方程，综合判别准确率为98.33%；（3）聚类分析显示：OJ与MJ群体的欧氏距离最小，HH与YZ群体的欧氏距离比OJ与MJ群体的要大，NLJ群体与其他5个群体的欧氏距离相对最大；（4）6群体间脂肪酸含量的变异系数在2.99%—30.00%之间，遗传多样性指数变化幅度在3.2732—3.3204之间。综上，基于脂肪酸生物标记法表明6水系绒螯蟹群体遗传多样性指数均较高，脂肪酸可以有效鉴别6水系野生绒螯蟹种群，整体上南流江群体和其余5群体间相距最远；本研究结果可为绒螯蟹种质资源评价和开发利用等提供基础资料。 |
| 关键词: 绒螯蟹属 地理群体 脂肪酸生物标记 遗传多样性 亲缘关系 |
| DOI：10.11693/hyhz20180900212 |
| 分类号:Q953;S968.2 |
| 基金项目:农业部现代农业产业技术体系专项资金项目，CARS-48号；上海市科委工程技术中心能力提升项目，16DZ2281200号；上海市教委水产动物遗传育种协同创新中心项目，A1-2041-18-0003号；深圳市澳华农牧有限公司横向项目，D-8006-16-0088号。 |
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| THE POPULATION INHERITANCE OF SIX MITTEN CRAB POPULATIONS (ERIOCHEIR SENSU STRICTO) IN COASTAL CHINA BASED ON PHOSPHOLIPID FATTY ACIDS BIOMARKERS |
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ZHANG Cheng1,2, ZU Lu1,2, LI Qing-Qing1,2, CHENG Yong-Xu1,2,3, WU Xu-Gan1,2,3
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1.Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture, Shanghai Ocean University, Shanghai 201306, China;2.Shanghai Collaborative Innovation Center for Aquatic Animal Genetics and Breeding, Shanghai Ocean University, Shanghai 201306, China;3.National Demonstration Centre for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China
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| Abstract: |
| The fatty acid composition of aquatic animal muscles can be used to evaluate their population inheritance. In this study, the fatty acid compositions of the six Eriocheir sensu stricto population muscles were measured and compared in phospholipid fatty acids biomarkers, and the six populations were from Liaohe, Huanghe, Changjiang (Yangtze), Oujiang, Minjiang, and Nanliujiang River basins. The genetic diversity and phylogenetic relationship were analyzed among the six Eriocheir s. s. populations. The results show as follows:(1) All the six Eriocheir s. s. populations contained 26 types of fatty acids among which the total volume of seven main fatty acids C20:5n3, C18:1n9, C22:6n3, C16:0, C20:4n6, C18:2n6, and C18:0 was over 74%; (2) There were eight fatty acid indices with high contribution to the discrimination of the six Eriocheir s. s. populations, including C17:0, C18:0, C20:0, C22:1n9, C18:3n3, C18:3n6, C20:2n6, and C22:5n3. Relevant discrimination equations were established for each Eriocheir s. s. population, the synthetic discriminant accuracy was 98.33%; (3) The results of cluster analysis show that the Euclidean distance between OJ and MJ populations were the closest, and the distance between HH and YZ populations were relatively larger than those of OJ and MJ populations, and the Euclidean distance of NLJ population was the farthest compared with the other five populations; (4) Among the six Eriocheir s. s. populations, the variable coefficients of fatty acid content ranged 2.99%-30.00%, and the genetic diversity indexes varied from 3.2732 to 3.3204. Overall, the genetic diversity indices of the six populations were quite high based on phospholipid fatty acids biomarkers. Fatty acids were effective to identify the six Eriocheir s. s. populations. The phylogenetic relationship of the other five populations were the farthest from NLJ population. The results of this study provide basic information for the evaluation, development and utilization of germplasm resources of Eriocheir s. s. |
| Key words: Eriocheir sensu stricto geographical population fatty acid biomarkers phylogenetic relationship genetic diversity |