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引用本文:闫茂仓,王瑶华,胡利华,张炯明,刘亚南,唐明,罗奎,黄贤克,周前进,陈炯.副溶血弧菌特异性卵黄抗体(AHPND-VpIgY)对凡纳滨对虾幼体被动免疫和育苗成活率的影响.海洋与湖沼,2019,(2):443-448.
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副溶血弧菌特异性卵黄抗体(AHPND-VpIgY)对凡纳滨对虾幼体被动免疫和育苗成活率的影响
闫茂仓1,2,3, 王瑶华2, 胡利华2, 张炯明2, 刘亚南2, 唐明2, 罗奎2, 黄贤克2, 周前进1,3, 陈炯1,3
1.宁波大学海洋学院 宁波 315832;2.浙江省海洋水产养殖研究所 浙江省近岸水域生物资源开发与保护重点实验室 温州 325005;3.应用海洋生物技术教育部重点实验室 宁波 315832
摘要:
将抗高致病性副溶血弧菌(Vibrio parahaemolyticus)特异性卵黄抗体(AHPND-VpIgY)应用于育苗生产,通过测定氨氮、亚硝酸盐、细菌总数、弧菌总数、相对保护率和育苗成活率,评价了AHPND-VpIgY在育苗期对凡纳滨对虾(Litopenaeus vannamei)幼体的保护效果。结果表明,0.1g/m3和0.2g/m3 AHPND-VpIgY组的相对保护率为35.7%和57.2%,两者均显著高于非特异性卵黄抗体组(P<0.05);两组的育苗成活率为50.8%和57.2%,比对照组分别提高19.1%和25.6%。各实验组氨氮、亚硝酸盐、细菌总数及弧菌总数与对照组无显著差异,对水质无影响。综上,特异性的AHPND-VpIgY能在不改变水质的前提下,有效提高凡纳滨对虾幼体的存活率、相对保护率和育苗成活率,从而提高凡纳滨对虾苗种产量和质量。
关键词:  凡纳滨对虾  副溶血弧菌  卵黄抗体  育苗期  保护效果
DOI:10.11693/hyhz20181200303
分类号:S968.22;S945;Q956
基金项目:浙江省自然科学基金项目,LY14C190006号;浙江省重大科技攻关项目,2016C02055-5号;宁波市科技创新团队项目,2015C110018号;浙江省重点研发项目,2015C03005号;国家农业产业技术体系,CARS-47号;浙江省科技计划项目,2016F10021号;宁波市江北区科技计划项目,2017B02号。
EFFECT OF SPECIFIC ANTI-VIBRIO PARAHAEMOLYTICUS IgY ON PASSIVE IMMUNOLOGY AND SURVIVAL RATE OF LITOPENAEUS VANNAMEI LARVAL
YAN Mao-Cang1,2,3, WANG Yao-Hua2, HU Li-Hua2, ZHANG Jiong-Ming2, LIU Ya-Nan2, TANG Ming2, LUO Kui2, HUANG Xian-Ke2, ZHOU Qian-Jin1,3, CHEN Jiong1,3
1.School of Marine Science, Ningbo University, Ningbo 315832, China;2.Zhejiang Key Laboratory of Exploitation and Preservation of Coastal Bio-resource, Zhejiang Mariculture Research Institute, Wenzhou 325005, China;3.Key Laboratory of Applied Marine Biotechnology of Ministry of Education, Ningbo 315832, China
Abstract:
We applied the specific egg yolk antibody of highly pathogenic Vibrio parahaemolyticus (AHPND-VpIgY) for seedling breeding of Litopenaeus vannamei. The protective effect with the antibody was evaluated by measuring water quality, total bacteria, total Vibrio count, relative protection rate, and seedling survival rate. The results show that the relative protection rate of the 0.1g/m3 and 0.2g/m3 AHPND-VpIgY groups was 35.7% and 57.2%, respectively, which is higher than that of the control (no AHPND-VpIgY) (P<0.05). The larva survival rate of the 0.1g/m3 and 0.2g/m3 AHPND-VpIgY groups was 50.8% and 57.2%, which was 19.1% and 25.6% higher than that of the control group, respectively. Meanwhile, no significant difference in ammonia nitrogen, nitrite, total bacteria, and total Vibrio count were found between the experiment groups and control group. Therefore, application of AHPND-VpIgY could increase the survival rate of the larva, the relative protection rate, and the survival rate of seedling, and enhance the yield and quality of L. vannamei seedlings while the water quality remained unchanged.
Key words:  Litopenaeus vannamei  Vibrio parahaemolyticus  egg yolk antibody  seedling stage  protective effect
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