| 摘要: |
| 为了探究球状伪镖水蚤群体的遗传多样性及其遗传结构，本研究以线粒体细胞色素氧化酶Ⅰ（mtCOⅠ）基因作为分子标记，提取了中国东南沿海地区5个采样点135只球状伪镖水蚤个体的基因组DNA并进行分析。结果表明：在获得的580bp的COⅠ基因序列中，碱基A+T的平均含量为63.4%，明显高于G+C的含量（36.6%），显示出较强的AT偏好性。5个采样群体共检测到49个多态位点和38个单倍型，单倍型多样性指数（h：0.6285—0.9214）和核苷酸多样性指数（π：0.00766—0.02010）均处于较高水平，各群体之间遗传距离为0.009—0.031。系统进化树和单倍型网络图显示：5个采样群体聚成多个支系；除广州与顺德、上海与无锡以外，其他各采样群体之间均具有显著遗传分化；5个采样群体可以划分为3个种群。中性检验和核苷酸不配对分布显示，各种群均未经历过种群扩张事件。 |
| 关键词: 桡足类 球状伪镖水蚤 mtCOⅠ 遗传多样性 遗传分化 |
| DOI：10.11693/hyhz20200300056 |
| 分类号:Q958 |
| 基金项目:国家重点研发计划资助项目，2018YFC1406301号；国家自然科学基金项目，41476114号；厦门市海洋经济发展专项资金项目，14CZY042HJ16号；自然资源部全球变化与海气相互作用专项（生物系统分类研究）。 |
附件 |
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| POPULATION GENETIC DIVERISTY AND GENETIC STRUCTURE OF PSEUDODIAPTOMUS FORBESI FROM THE COASTAL AREAS OF SOUTHEAST CHINA |
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WANG Long-Sheng1, LI Hao-Ran1, GUO Dong-Hui1,2,3,4
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1.College of Ocean and Earth Sciences, Xiamen University, Xiamen 361102, China;2.Marine Biodiversity and Global Change Research Center, Xiamen 361102, China;3.Xiamen City Key Laboratory of Urban Sea Ecological Conservation and Restoration, Xiamen 361102, China;4.Fujian Provincial Key Laboratory for Coastal Ecology and Environmental Studies, Xiamen University, Xiamen 361102, China
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| Abstract: |
| To investigate the genetic diversity and populations structure of Pseudodiaptomus forbesi, 135 samples, from five areas across the southeastern of China, were collected and analyzed using the mitochondrial marker cytochrome oxidase subunit Ⅰ (mtCOⅠ). The results show that the average contents of A+T (63.4%) were significantly higher than those of G+C (36.6%) in the 580bp fragment, indicating a preference for A and T base. Forty-nine polymorphic sites and 38 haplotypes were detected from the five geographic assemblages. All assemblages had high haplotype diversity (h: 0.6285—0.9214) and nucleotide diversity (π: 0.00766—0.02010). The genetic distances among five assemblages ranged from 0.009 to 0.031. Based on phylogeny and haplotype network, five assemblages were separated into several lineages. The pairwise fixation index (Fst) and gene flow (Nm) show that genetic differentiation was extremely significant between all assemblages except for Guangzhou and Shunde, Shanghai and Wuxi assemblages. The five assemblages could be divided into three populations. Neutrality tests and mismatch distribution analysis inferred population expansion had not taken place in all populations. |
| Key words: Copepod Pseudodiaptomus forbesi mtCOⅠ genetic diversity genetic differentiation |
