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引用本文:丛岩懿,谢玉素,张留所.海洋线虫Litoditis marina酸性pH胁迫响应的转录组分析.海洋与湖沼,2020,51(6):1472-1482.
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海洋线虫Litoditis marina酸性pH胁迫响应的转录组分析
丛岩懿1,2,3,4, 谢玉素1,2,3, 张留所1,2,3
1.中国科学院海洋研究所 中国科学院实验海洋生物学重点实验室 青岛 266071;2.青岛海洋科学与技术试点国家实验室海洋生物学与生物技术功能实验室 青岛 266237;3.中国科学院海洋大科学研究中心 青岛 266071;4.中国科学院大学 北京 100049
摘要:
工业革命以来,二氧化碳排放导致了海洋酸化。海水pH下降对无脊椎动物的生长发育、繁殖、代谢和免疫等多个生命过程产生重要影响,但海洋无脊椎动物如何感知和响应酸性pH胁迫的分子机制还很不清楚。本研究以团队建立的海洋线虫Litoditis marina品系为模型,对其在不同酸性pH条件下的表型特征及转录组数据进行了分析。结果表明,当pH从实验室最佳生长条件5.92下降到5.33时,L. marina生长发育的速度明显减慢,但依然可以产卵繁殖;但当pH下降到4.33时,L. marina的生长受到严重影响,表现为不能长成成体且不能产卵繁殖。通过转录组数据分析,发现当pH从5.92下降到4.33时,海洋线虫L. marina脂肪酸β-氧化通路基因和不饱和脂肪酸合成相关基因表达上调;表皮相关基因表达则呈现差异变化,2个nas基因和6个ptr基因表达显著上调,表皮胶原基因col类基因的表达没有发生显著变化;细胞色素P450通路相关基因、凝集素C基因和HSP70家族基因的表达量显著上调。当pH从5.92下降到5.33时,上述提到的这些上调基因中的大多数没有发生显著变化。我们发现的海洋线虫应答酸化胁迫的主要调控模式,为理解生物体能够在低pH环境中生存的分子机制提供了参考,为筛选和识别生物体响应和适应酸化胁迫的关键基因奠定了基础。
关键词:  海洋线虫  Litoditis marina  酸性pH胁迫  脂肪酸β-氧化  物质代谢细胞色素P450通路
DOI:10.11693/hyhz20200300070
分类号:
基金项目:青岛海洋科学与技术试点国家实验室山东省重大科技创新工程课题,2018SDKJ0302-1号;青岛海洋科学与技术试点国家实验室海洋生物学与生物技术功能实验室2018年度青年科研人员特别资助项目,2018.09-2020.08;青岛创业创新领军人才项目,Grant 16-8-3-19-zhc号;中国科学院海洋大科学研究中心重点部署项目,2019.11—2022.11。
TRANSCRIPTOME ANALYSIS OF THE RESPONSE OF MARINE NEMATODE LITODITIS MARINA TO ACIDIC STRESS
CONG Yan-Yi1,2,3,4, XIE Yu-Su1,2,3, ZHANG Liu-Suo1,2,3
1.CAS Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;2.Laboratory for Marine Biology and Biotechnology, Pilot National Laboratory for Marine Science and Technology(Qingdao), Qingdao 266237, China;3.Center for Ocean Mega-Science, Chinese Academy of Sciences, Qingdao 266071, China;4.University of Chinese Academy of Sciences, Beijing 100049, China
Abstract:
Since the industrial revolution, carbon dioxide emissions have led to ocean acidification. The decrease in pH of seawater has important effects on the growth, reproduction, metabolism, and immune regulation of organisms. At present, the molecular mechanisms by which marine organisms perceive and respond to acidic stress remain largely unknown. In this study, marine nematode Litoditis marina was used as an animal model to analyze its phenotypic and transcriptome characteristics under different pH values. Our report provided a reference for exploring the regulatory mechanism of marine invertebrates in response to the acidic stress of seawater. We found that when pH decreased from 5.92 to 5.33 (the optimum growth condition of L. marina is 5.92 in laboratory), the growth and development rate of L. marina decreased significantly, but it could still spawn. In contrast, when pH dropped to 4.33, the growth of L. marina was seriously affected, showing that it could not grow into adulthood and spawn. Through transcriptome analysis, we found that when pH decreased from 5.92 to 4.33, the expression of the fatty acid β-oxidation pathway genes and the unsaturated fatty acid synthesis genes were significantly up-regulated in L. marina. Furthermore, the expressions of 2 nas genes and 6 ptr genes were also significantly up-regulated, while the expression level of col genes were not changed. Additionally, we found that cytochrome P450 pathway genes, lectin C genes and HSP70 family genes were significantly up-regulated. However, when pH decreased from 5.92 to 5.33, most of the above up-regulated genes did not change significantly. This study may lay a foundation for the future study for identifying the master gene(s) responding and adaptation to an acidic stress in nematodes and other marine animals.
Key words:  marine nematodes  Litoditis marina  acidic stress  fatty acid β-oxidation  drug metabolism cytochrome P450
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