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引用本文:赵海霞,王学颖,杜腾飞,周莉,徐世宏,王彦丰,肖永双,刘清华,李军.体内受精许氏平鲉(Sebastes schlegelii)和体外受精大菱鲆(Scophthalmus maximus)精子结构及生理特性比较.海洋与湖沼,2020,51(6):1513-1519.
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体内受精许氏平鲉(Sebastes schlegelii)和体外受精大菱鲆(Scophthalmus maximus)精子结构及生理特性比较
赵海霞1,2,3, 王学颖1,2, 杜腾飞1,2,3, 周莉1,2,3, 徐世宏1,2,4, 王彦丰1,2, 肖永双1,2, 刘清华1,2, 李军1,2
1.中国科学院海洋研究所 实验海洋生物学重点实验室 海洋大科学研究中心 青岛 266071;2.青岛海洋科学与技术国家实验室海洋生物学与生物技术功能实验室 青岛 266237;3.中国科学院大学 北京 100049;4.1. 中国科学院海洋研究所 实验海洋生物学重点实验室 海洋大科学研究中心 青岛 266071
摘要:
为了解硬骨鱼许氏平鲉精子体内受精以及精子在雌性体内保持长期存活的特性,本研究应用扫描电镜与透射电镜对体内受精卵胎生硬骨鱼许氏平鲉与体外受精卵生大菱鲆的精子进行超微结构观察,并使用计算机精子分析系统比较精子激活后运动参数差异。结果显示,这两种鱼的精子均由头部,中段和尾部构成,无顶体,尾部轴丝为“9+2”微管结构。许氏平鲉精子特点:头部呈短棒状,细胞核呈长锥状;中段不对称,约30—40个线粒体呈垛叠状紧密排列在中段;轴丝质膜延伸形成的侧鳍较为发达。大菱鲆精子特点:头部呈椭圆形,头部和鞭毛结构松散,含有大量囊泡状结构;精子中段较短,向外突出的线粒体包裹鞭毛形成“半袖套”结构且尾部鞭毛侧鳍不发达。许氏平鲉精子密度和体外激活寿命均显著高于大菱鲆精子,而激活后精子的平均曲线速度(VCL)、平均直线运动速度(VSL)、平均路径速度(VAP)显著低于大菱鲆精子。研究表明,上述精子结构差异主要表现在精子头部与中段:体内受精硬骨鱼精子大多具有细长的头部,发达的中段,线粒体数量较多;体外受精硬骨鱼具有圆形或椭圆形的头部,中部不明显,线粒体较少。
关键词:  许氏平鲉  大菱鲆  精子超微结构  体内受精  体外受精
DOI:10.11693/hyhz20200300087
分类号:Q492;S917
基金项目:国家自然科学基金项目,31802278号;国家重点研发计划,2018YFD0901205号;现代农业产业技术体系项目,CARS-47号。
SPERM ULTRASTRUCTURAL AND PHYSIOLOGICAL DIFFERENCES BETWEEN INTERNALLY FERTILIZED BLACK ROCKFISH (SEBASTES SCHLEGELII) AND EXTERNALLY FERTILIZED TURBOT (SCOPHTHALMUS MAXIMUS)
ZHAO Hai-Xia1,2,3, WANG Xue-Ying1,2, DU Teng-Fei1,2,3, ZHOU Li1,2,3, XU Shi-Hong1,2,4, WANG Yan-Feng1,2, XIAO Yong-Shuang1,2, LIU Qing-Hua1,2, LI Jun1,2
1.The Key Laboratory of Experimental Marine Biology, Center for Ocean Mega-Science, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;2.Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266237, China;3.University of Chinese Academy of Sciences, Beijing 100049, China;4.1. The Key Laboratory of Experimental Marine Biology, Center for Ocean Mega-Science, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China
Abstract:
To understand the characteristics of sperm internal fertilization and the long-term survival in the body, the sperm of black rockfish and turbot were observed by scanning and transmission electron microscope, and the computer-assisted sperm analysis (CASA) system was utilized for comparing the differences of kinetic parameters after activation. The results show that the spermatozoa of black rockfish and turbot were composed of head that were devoid of acrosome, midpiece, and the tail showing “9+2” microtubules. In black rockfish, the head was rod-shaped with long conical nucleus. The midpiece was asymmetrical and consisted of 30 to 40 mitochondria closely arranged in stacks. In turbot, the oval head and flagella were loosely arranged with many vesicles. Ten to twenty mitochondria kept inside and formed “half- sleeve”. The lateral fin was shorter. The sperm density and life span of black rockfish were significantly higher than those of turbot sperm, while the average curve-line speed (VCL), straight-line velocity (VSL) and average path velocity (VAP) were lower significantly. Research suggests that the sperm of internal fertilized teleost had longer head, longer midpiece contained with a large number of mitochondria; the sperm of external fertilized teleost had oval head, the midpiece was shorter and less quantity in mitochondria.
Key words:  Sebastes schlegelii  Scophthalmus maximus  spermatozoa ultrastructure  internal fertilization  external fertilization
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