| 摘要: |
| 本研究利用选择培养基从鳗鲡精养殖池和循环水水处理系统中分离筛选出二株脱氮细菌，应用16S rRNA分子生物学鉴定，确定菌株的种属。菌株NB-1属于芽孢杆菌属（Bacillus）、菌株DB-1是恶臭假单胞菌（Pseudomonas putida）。对菌株硝化及反硝化作用性能的研究结果表明：菌株NB-1具有良好的亚硝化及硝化作用性能，在投菌量为0.025%时，氨氮降解率为69.5%，亚硝酸盐氮降解率为99.2%。菌株DB-1在厌氧条件下表现出良好的反硝化作用性能，最适投菌量为0.6%，最佳C/N为12，对硝酸盐氮的降解率为94.6%，对总氮的降解率为64.0%。因此本研究筛选出的二株脱氮细菌可以广泛应用于养殖水体水质调控，具有良好的市场应用前景。 |
| 关键词: 硝化作用 反硝化作用 C/N 功能菌株 |
| DOI：10.11693/hyhz20200400106 |
| 分类号:S949;S955 |
| 基金项目:国家重点研发计划“池塘尾水生态治理技术及工程设施研发”项目，2019YFD0900302号；福建省科技厅区域发展项目，2016N3002号。 |
附件 |
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| ISOLATION AND SCREENING OF HIGH-EFFICIENCY NITRIFICATION AND DENITRIFICATION FUNCTIONAL STRAINS AND EFFECT EVALUATION |
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GUO Shao-Peng1,2, JIANG Xing-Long1,2, WANG Ze-Xu1,2, WEI Jin-Sheng1,2
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1.Fisheries College, Jimei University, Xiamen 361021, China;2.Engineering Research Center of the Modern Technology for Eel Industry, Ministry of Education, Xiamen 361021, China
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| Abstract: |
| Two strains of nitrification and denitrification functional strains were isolated and screened from eel culture ponds and recirculating water treatment system by selective medium, and the selected strains were determined by 16S rRNA molecular biological identification. NB-1 strain was belonged to the genus Bacillus, DB-1strain belonged to the genus Pseudomonas putida. The results show that NB-1 strain had good nitrosation and nitrification performance, and the degradation rate of ammonia nitrogen and nitrite were 69.5% and 99.2% respectively, where the ratio of NB-1 strain was 0.025%. DB-1 strain showed good denitrification performance under anaerobic condition. The optimal dosage ratio of DB-1 strain was 0.6%, and the optimal C/N ratio was 12, where the degradation rate of nitrate was 94.6% and that of total nitrogen was 64.0%. Therefore, the two strains would be widely used in aquaculture to improve water quality and have good application potential. |
| Key words: nitrification denitrification functional strains C/N |
