| 引用本文: | 李伟业,徐志进,殷小龙,陈爽,马雪彬,章霞,邱豪军,张晓林,蒋霞敏.温度对美洲黑石斑(Centropristis striata)幼鱼生长和免疫因子活力与相关基因表达的影响.海洋与湖沼,2021,52(3):708-717. |
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| 摘要: |
| 采用温度渐变的方法，研究了18、22、26、30℃共4个温度梯度对美洲黑石斑幼鱼[（73.74±3.76）g]生长及免疫因子活力的影响。结果表明，温度对美洲黑石斑幼鱼的生长和存活均有不同程度的影响。在实验温度范围内，随着温度的升高，美洲黑石斑幼鱼最终体重、特定增长率（SGR）、日增重（DWG）、增重率（GBW）和增长率（GBL）均出现先升高后降低的趋势，且部分温度组间差异显著（P<0.05），其中上述各项指标中，温度22℃组均最高，与温度26℃组差异不显著（P>0.05），而与温度18℃、30℃组存在显著性差异（P<0.05）；幼鱼的饲料系数（FCR）随温度升高先降低后升高，且部分温度组间差异显著（P<0.05）。在成活率方面，除温度30℃组的成活率为82.2%，显著低于其他温度组外（P<0.05），其他各温度组成活率均达到90%以上。温度对美洲黑石斑幼免疫相关酶活力和基因表达也存在一定影响。在实验第1 d时，碱性磷酸酶（AKP）、酸性磷酸酶（ACP）和溶菌酶（LSZ）活力随温度的升高呈逐渐升高的趋势，而在实验第60 d时，碱性磷酸酶（AKP）、酸性磷酸酶（ACP）和溶菌酶（LSZ）活力随温度升高呈先升高后降低的趋势，且22℃和26℃组显著高于18℃和30℃组（P<0.05）；而超氧化物歧化酶（SOD）活力在实验第1 d时随温度的升高呈先下降后升高的趋势，实验第60 d时超氧化物歧化酶（SOD）活力随温度的升高逐渐上升。温度也影响HSP70和HSP90基因的表达，尽管实验期间各实验组HSP70和HSP90基因的表达变化未呈现明显的规律性，但30℃组HSP70和HSP90的表达量显著高于其他组（P<0.05）。从以上结果可见，温度对美洲黑石斑幼鱼的生长和免疫因子活力有一定影响，综合实验鱼的生长和免疫因子实验数据，美洲黑石斑幼鱼最适的生长温度应为22—26℃。 |
| 关键词: 温度 美洲黑石斑 生长 免疫酶 HSP70 HSP90 |
| DOI：10.11693/hyhz20200900257 |
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| 基金项目:浙江省重点研发计划项目，2019C02048号；舟山市公益类科技项目，2019C31053号。 |
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| EFFECTS OF TEMPERATURE ON GROWTH, IMMUNE FACTOR ACTIVITY AND RELATED GENE EXPRESSION OF JUVENILE CENTROPRISTIS STRIATA |
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LI Wei-Ye1,2, XU Zhi-Jin2, YI Xiao-Long2, CHEN Shuang2, MA Xue-Bin2, ZHANG Xia2, QIU Hao-Jun2, ZHANG Xiao-Lin3, JIANG Xia-Min1
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1.School of Marine Science, Ningbo University, Ningbo 315832, China;2.Zhoushan Fisheries Research Institute of Zhejiang Province, Zhoushan 316000, China;3.Zhejiang Ocean University, Zhoushan 316022, China
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| Abstract: |
| Using the temperature gradient method, we studied The effects of temperature gradients of 18, 22, 26, and 30℃ on growth and immune factor activity of juvenile Centropristis striata[(73.74±3.76) g]. The results indicate that temperature affected the growth and survival of the juveniles in a different degree. With the increase of temperature, the final body weight, final body length, specific growth rate (SGR), daily weight gain (DWG), weight gain rate (GBW) and growth rate (GBL) of the juvenile fish showed a trend of first increasing and then decreasing, but significant differences were shown among groups (P<0.05). The 22℃ group showed the best performance in all the above indicators so did similarly in group at 26℃, but the 18℃ and 30℃ groups. The feed coefficient of juvenile C. striata decreased first and then increased with the increase of temperature, and the among-group difference was significant (P<0.05). In survival rate, the 30℃ group was the lowest (82.2%), which was significantly lower than those of other groups were (P<0.05) (>90%). Temperature had also a certain effect in terms of the activity of immune-related enzymes and gene expression in the juveniles. The activities of alkaline phosphatase (AKP), acid phosphatase (ACP) and lysozyme (LSZ) gradually increased with the temperature on the 1th Day of the experiment, and first increased and then decreased with the temperature increasing on the 60th day of the experiment. Moreover, the above-mentioned activities in 22℃ and 26℃ groups were significantly higher than those in 18℃ and 30℃ groups (P<0.05). The activity of superoxide dismutase (SOD) decreased first and then increased with the increase of temperature on the 1th day of the experiment, and gradually increased with the increase of temperature on the 60th day of the experiment. Temperature also affected the expressions of HSP70 and HSP90. Although the expressions in each experimental group did not show obvious regularity, the expression levels in the 30℃ group were significantly higher than those in other groups (P<0.05). Therefore, temperature affected obviously the growth and immune factor activity of juvenile C. striata. The experimental data show that the optimal growth temperature of juvenile C. striata was 22-26℃. |
| Key words: temperature Centropristis striata growth immune enzyme HSP70 HSP90 |
