引用本文:	邓远,李振通,苏金枝,黎琳琳,邱弈树,丁小雨,段鹏飞,王心怡,王林娜,刘阳,马文辉,赵侠,李胜忠,田永胜.金虎杂交斑三倍体血细胞特征及染色体核型分析.海洋与湖沼,2024,55(2):509-516.

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金虎杂交斑三倍体血细胞特征及染色体核型分析
邓远,李振通,苏金枝,黎琳琳,邱弈树,丁小雨,段鹏飞,王心怡,王林娜,刘阳,马文辉,赵侠,李胜忠,田永胜
1.新疆农业大学生命科学学院新疆乌鲁木齐 830052;2.农业农村部海洋渔业可持续发展重点实验室中国水产科学研究院黄海水产研究所山东青岛 266071;3.中国水产科学研究院海南创新研究院海南三亚 572000;4.莱州明波水产有限公司山东莱州 261400

摘要:

为了解三倍体金虎杂交斑(棕点石斑鱼Epinephelus fuscoguttatus♀×蓝身大斑石斑鱼E. tukula♂)的细胞遗传特性。以静水压休克诱导的三倍体金虎杂交斑为研究对象, 通过细胞DNA含量测定鉴定出三倍体金虎杂交斑、对三倍体金虎杂交斑进行红细胞大小、形态的比较及染色体核型分析。结果显示, 三倍体金虎杂交斑的DNA含量与二倍体金虎杂交斑的DNA含量的比值为1.45︰1。三倍体金虎杂交斑血细胞长轴、短轴分别为二倍体的1.34、1.14倍(P<0.01)。三倍体金虎杂交斑血细胞体积、表面积分别为二倍体的2.07和1.54倍(P<0.01), 三倍体金虎杂交斑血细胞核长轴、短轴、核表面积、核体积分别为二倍体的1.22、1.05、1.29、1.57倍(P<0.01)。通过头肾-秋水仙素注射法进行染色体核型分析结果显示, 二倍体金虎杂交斑的染色体数目为48, 核型为2n=48=2sm+46t, NF=50, 三倍体金虎杂交斑的染色体数目为72, 核型为3n=72=1m+2sm+69t, NF=75, 未发现有性染色体。实验结果为石斑鱼杂交种多倍体育种提供了丰富生物学基础数据。

关键词: 金虎杂交斑红细胞形态流式细胞术三倍体二倍体染色体核型

DOI：10.11693/hyhz20231100243

分类号:S917.4

基金项目:“十四五”国家重点研发计划,2022YFD2400502号;泰山产业领军人才项目,LJNY202109号;山东省重点研发计划项目,2022LZGC016号;海南省重点研发项目,ZDYF2022XDNY243号;财政部和农业农村部:国家现代农业产业技术体系,CARS-47-G31号;中央公益性科研院所基本科研业务费,20603022024013号。

附件

BLOOD CELL CHARACTERISTICS AND CHROMOSOMAL KARYOTYPE ANALYSIS OF TRIPLOID HYBRID GROUPER

DENG Yuan^1,2,3, LI Zhen-Tong^2,3,4, SU Jin-Zhi^1,2,3, LI Lin-Lin^2,3,4, QIU Yi-Shu^1,2,3, DING Xiao-Yu^1,2,3, DUAN Peng-Fei^2,3, WANG Xin-Yi^2,3, WANG Lin-Na^2,3, LIU Yang^2,3,4, MA Wen-Hui⁵, ZHAO Xia⁵, LI Sheng-Zhong¹, TIAN Yong-Sheng^2,3,4

1.College of Life Science, Xinjiang Agricultural University, Urumqi 830052, China;2.Key Laboratory of Sustainable Development of Marine Fisheries, Ministry of Agriculture and Rural Affairs;3.Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China;4.Hainan Innovation Research Institute, Chinese Academy of Fishery Sciences, Sanya 572000, China;5.Mingbo Aquatic Co., Ltd., Laizhou 261400, China

Abstract:

In order to understand the cytogenetic characteristics of the triploid hybrid grouper (Epinephelus fuscoguttatus♀×E. tukula♂), this experiment focused on the triploid hybrid grouper induced by hydrostatic pressure shock as the research object. The experiment identified the triploid hybrid grouper by determining DNA content of the cells, compared the size and morphology of the red blood cell of the triploid hybrid grouper, and performed karyotype analysis of the chromosomes. The results showed that the DNA content ratio between triploid and diploid hybrid groupers was 1.45:1. The long and short axes of the blood cells in the triploid hybrid grouper were 1.34 and 1.14 times those of the diploid (P<0.01). The volume and surface area of the blood cells in the triploid hybrid grouper were 2.07 and 1.54 times those of the diploid (P<0.01), and the long and short axes of the nucleus, as well as the nucleus surface area and volume of the triploid hybrid grouper were 1.22, 1.05, 1.29, and 1.57 times those of the diploid (P<0.01). Karyotype analysis using the head kidney-colchicine injection method showed that the diploid hybrid grouper had 48 chromosomes, with a karyotype of 2n=48=2sm+46t, NF=50, while the triploid hybrid grouper had 72 chromosomes, with a karyotype of 3n=72=1m+2sm+69t, NF=75, and no sex chromosomes were found. The experimental results provide rich biological data for the breeding of multiple ploidies in hybrid groupers.

Key words: hybrid grouper red blood cell morphology flow cytometry triploid diploid chromosomal karyotype