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钝顶螺旋藻质粒的电镜观察及杂交研究
秦松1, 童顺1, 崔武2, 李亚菲3, 王希华1, 武建秋1, 吴光耀2, 张培军1, 曾呈奎1
1.中国科学院海洋研究所 青岛 266071;2.北京大学生物系 北京 100871;3.中国人民解放军肝病研究所 北京 100700
摘要:
于1992年6月－1993年4月，对钝顶螺旋藻质粒进行透射电镜观察及分子杂交研究。结果表明，利用单层分子膜展开技术，能够观察到超螺旋以及闭合环形的质粒图象；S6质粒大小的平均值约为2.0kb，与电泳检测的结果稍有出入。斑点杂交结果表明，不同形态藻株S6、F3、F3弯与F3直质位之间具有一定同源性，与染色体DNA之间均显示同源性。对钝顶螺旋藻质位的进一步研究将推动其基因工程育种的开展。
关键词:  蓝藻  钝顶螺旋藻  质粒
DOI：
分类号:
基金项目:中国科学院海洋研究所调查研究报告第2338号。中国科学院院长基金部分资助，中国科学院海洋研究所所长基金资助
TEM OBSERVATION AND HYBRIDIZATION STUDY OF PLASMID FROM SPIRULINA PLATENSIS
Qin Song1, Tong Shun1, Cui Wu2, Li Yafei3, Wang Xihua1, Wu Jianqiu1, Wu Guangyao2, Zhang Peijun1, Zeng Chengkui (C. K. Tseng)1
1.Institute of Oceanology Academia Sinica, Qingdao 266071;2.Department of Biology, Beijing University, Beijing 100871;3.Institute for Liver Diseases, PLA, Beijing 100700
Abstract:
Transmission electron microscopic observation and hybridization study of the plasmid from Spirulina platensis were carried out from June, 1992 to April, 1993. Supercoiled and closed circular patterns were observed under a transmission electron microscope. The size of the plasmid from S6 strain was about 2.0kb, which was slightly different from the result of electrophoresis. Results of dot blotting show that plasmids from strains of S6, F3, F3 spiral and F3 straight differing in form are homologous to each other and are also homologous to chromosomal DNAs.
Key words:  Cyanophyte, Spirulina platensis, Plasmid
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