| 摘要: |
| 于1991年7月-1992年7月在浙江省宁波、舟山两市暴发了中国对虾肠结节病。在宁海等地对虾养殖场，采集患肺结节病中国对虾，运用电镜观察患病中国对虾鳃丝、肝胰腺、中肠、肌肉等组织，检查枝原体的存在与否及其主要病理变化。结果表明，病虾近肠壁表皮细胞细胞质和核周腔有枝原体侵入。细胞质内枝原体形态大小不一，近卵形的直径为0.12-1.2 μm；弯条状的直径约0.09 μm，长度均在0.25-1.4μm。核周腔内枝原体均为卵形，直径约0.12-0.16 μm。枝原体无细胞壁，仅有一层膜包围，膜厚约8nm，膜上均匀地沉着电子致密颗粒；枝原体细胞中部电子密度较浅；细胞质中丝状枝原体形成分枝并在其分枝顶端膨大成球状；核周腔异常膨胀，核明显变形，有多处缢痕，枝原体以裂殖和芽殖方式分裂子代。枝原体感染引发的肠道局部肿胀甚至梗塞是导致该发病地区中国对虾死亡的主要原因。 |
| 关键词: 中国对虾 枝原体 超微结构 细胞病理 |
| DOI： |
| 分类号: |
| 基金项目:国际科学基金组织（IFS）资助，A／2199－1号 |
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| MYCOPLASMA ULTRASTRUCTURE OF PENAEID SHRIMP (PENAEUS CHINENSIS) AND MAJOR ULTRAPATHOLOGIC CHANGES OF HOST CELLS |
|
Yang Jifang
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Second Institute of Oceanography, SOA, Hangzhou 310012
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| Abstract: |
| The ultrastructure of parasitic organism (mycoplasma) in diseased penaeid shrimp and cell-pathologic changes of host are reported in this paper. A hut-node disease of shrimp broke out in shrimp culture-ponds in the east coast of Zhejiang Province in summer of 1991 and 1992. TEM observation showed that mycoplasmas invade cytoplasma and the perinuclear space of epithelium cells near the gut wall. Mycoplasma was found to be variable in shape from spherical structure (0.12 - 1.2 μm in diameter) to slenderly branched ?lameter of uniform diameter (about 0.09μm), ranged from 0.25 to 1.4 μm in length in cytoplasma of the host cell. Generally, the mycoplasma in the perinuclear space is a spherical structure (from 0.12 to 0.16 μm in diameter). There is no intrusion of mycoplasma into the nuclear substance. The mycoplasma has no cell wall but is enclosed by a membrane layer (8 nm in thickness) on which are a lot of high electron density particles. There is an empty vacuole in the centre of some mycoplasmas. Filameter mycoplasma forms branches, whose end expands into a spherical structure. The host cell nuclear changed obviously into tie-shaped multiploid with multi-constriction. The nuclear pore is destroyed seriously. The mycoplasma multiplies during splitting and sprouting. |
| Key words: Penaeus chinensis, Mycoplasma, Ultrastructure, Cell pathology |
