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环境因子对三种常见微藻细胞中二甲基硫丙酸含量影响的初步研究
李炜, 焦念志
中国科学院海洋研究所 青岛266071
摘要:
以3种常见的海洋浮游藻类—扁藻、杜氏藻和牟氏角刺藻作为实验材料,采用正交方法设计实验条件,研究盐度、温度和光强的变化对藻类细胞内二甲基硫丙酸(DMSP)含量的影响。结果表明,3种藻类细胞DMSP含量相差很大。扁藻DMSP含量最高,其次为杜氏藻,牟氏角刺藻含量最低。种间差别的影响明显高于环境条件的变化对藻细胞DMSP含量的影响。3种环境因子对藻细胞DMSP含量的影响效果不同,盐度变化引起藻细胞DMSP含量的变化最为显著,其次为温度和光强。随着盐度的增加,扁藻、杜氏藻和牟氏角刺藻细胞内DMSP含量均呈上升趋势。
关键词:  藻细胞  二甲基硫丙酸  温度  盐度  光强
DOI:
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基金项目:国家自然科学基金资助项目,39625008号,39630060号
EXPERIMENTAL STUDIES ON ENVIRONMENTAL FACTORS REGULATING INTRACELLULAR DIMETHYLSULPHONIOPROPIONATE OF THREE UNICELLULAR ALGAE SPECIES
LI Wei, JIAO Nian-Zhi
Institute of Ocaenology, The Chinese Academp of Scieiences, Qingdao, 266071
Abstract:
Three unicellular algae species, Platymonas spp., Dunaliella spp. and Chaetoceros muelleri, were selected for lab culture to investigate the influence of salinity (10–80), temperature (18.0–28.0 °C) and light (1.020–3.104 Mw/cm2) on the intracellular dimethylsulphoniopropionate (DMSP) content. The results show that the cellular DMSP contents of these three algae were different, with Platymonas spp. being the highest and Chaetoceros muelleri the lowest. The differences in cellular DMSP content among different species were larger than the differences caused by environmental factors on the same species under the experimental conditions. Among the three environmental factors, the influence of salinity was the most significant, followed by temperature. Light, as a basic ecological factor, showed little effect on the production of DMSP. The intracellular DMSP contents of all these algae increased with increasing salinity of the media, but the degree of increase differed among different species.
Key words:  Algae cells, DMSP, Salinity, Temperature, Light
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