| 摘要: |
| 采用在人工模拟潮间带生态环境中养殖多年的龙须菜藻体，经70%乙醇、1%NaCLO或低光等逆境胁迫后，培养新生直立枝，研究匍匐体类愈伤组织的诱导及机制。结果表明，刷落2–4mm长的新生直立枝培养30d后，基部出现由丛生丝状体组成的类愈伤组织。丝状体能够固着、分化，不断分裂形成匍匐体。这是除孢子外，首次从江篱体细胞获得有固着能力的再生苗。该再生苗弥补了营养枝繁殖中须人工固着的缺点。ELISA测定内源激素含量结果表明：在不同胁迫下，藻体的不同部位内源激素含量发生改变，尤其是ABA的积累与高等植物在逆境胁迫中表现相同，因而推测类愈伤组织产生及快速分化可能与海藻经逆境胁迫后内源激素含量变化、信息传递有关。 |
| 关键词: 龙须菜 类愈伤组织 匍匐体 内源激素 逆境胁迫 |
| DOI：10.11693/hyhz200001005005 |
| 分类号: |
| 基金项目:山东省自然科学基金,2914 |
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| INDUCTION OF CALLUS-LIKE TISSUE FROM CREEPING TISSUE OF GRACILARIA LEMANIFORMIS (RHODOPHYTA) AND MECHANISM ANALYSIS |
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FAN Yang, LI Ren-zhi
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Institute of Oceanology, The Chinese Academy of Sciences, Qingdao, 266071
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| Abstract: |
| Gracilaria lemaniformis has been cultured in a tank simulating the intertidal environment for three years. After the alga with erect thalli and creeping tissue was treated with three kinds of stress: 70% ethanol for 15s, 1%NaCLO for 15min and low light intensity, regenerated erect thalli (2–4mm) were separated from creeping tissue and cultured for 30d. Callus-like tissue composed of tufted filaments appeared from the bottom of these thalli. These filaments were able to attach, differentiate, divide, and finally grow into creeping tissue. The obtained seedlings with creeping tissue from callus-like tissue of G. lemaniformis, compared with sporeling, have characteristics of fast growth and anti-epiphyte. This is a new method to resolve seedling resource of Grcilaria culturation. The results of ELISA show that the in vivo hormone level of erect thalli and creeping tissue changed in response to stress. Especially, the increase of ABA was just the same as higher plants. In conclusion, it is possible that the forming of callus-like tissue and its differentiation are related to the in vivo hormone level. |
| Key words: Gracilaria lemaniformis, Callus-like tissue, Creeping tissue, Hormone, Stress |
