| 摘要: |
| 于1997年在香港科技大学，采用LACHAT自动离子分析仪对裙带菜雄配子体（1992年6月自青岛分离的野生品系）进行营养分析。结果表明，在恢复营养供给后的1h内，雄配子体对NH4-N、PO4-P和NO3-N三种营养盐的吸收速度依次为22.71μg/(g*h)、14.86μg/(g*h)和8.08μg/(g*h)，吸收速度的排列顺序不随培养时间的延长而变化；雄配子体对氨氮的吸收速度远高于对硝酸氮的吸收速度，饥饿的雄配子体细胞对三种营养盐的吸收均表现出短时、快速的特点。本文还依据在丰富培养过程中利用AODC计数的总异养菌数的变化特点，对可能造成的影响进行了初步的讨论. |
| 关键词: 裙带菜 雄配子体 营养吸收 |
| DOI：10.11693/hyhz200001006006 |
| 分类号: |
| 基金项目:国家“863”计划资助,863－819－03－01 |
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| NUTRIENT UPTAKE IN SUSPENSION CULTURE OF VEGETATIVE MALE GANETOPHYTE OF UNDARIA PINNATIFIDA |
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PANG Shao-jun, XIAO Tian
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Institute of Oceanology, The Chinese Academy of Sciences, Qingdao, 266071
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| Abstract: |
| Multiplication of vegetative gametophytes of Undaria pinnatifida is one of the important procedures in the recently established clone techniques to produce juvenile sporelings. Kinetics of nutrient uptake is a decisive factor to determine how often the medium should be changed. Under favorable culture conditions, the relative growth rate of vegetative growth rate is around 20%, therefore it is necessary to understand the related process. Results of dynamics of nutrient uptake (NH4-N, PO4-P and NO3-N) by vegetative gametophytes of Undaria pinnatifida are reported in this paper. The male gametophyte was isolated in 1992 in Qingdao, China, and maintained under dim light to keep its vegetative state. For nutrient uptake analyses, the filamentous gametophyte were transferred to high irradiance and temperature in pure sterilized seawater for three days. Nutrient analyses were completed by automatic ion analyzer (LACHAT) by analyzing the water samples taken at certain time interval and stored at minus 80 degree centigrade. The uptaking rates of NH4-N, PO4-P and NO3-N within the first hour were 22.71, 14.86 and 8.08 μg/(g*h), respectively. The order of uptaking rates afterwards among three nutrients did not change. The uptaking rates of NH4-N was all the time much higher than that of NO3-N. The uptaking rate of PO4-P was also much higher than expected. The amount that the vegetative gametophyte uptook within the hour was roughly equal to the total amount for the rest five hours thereafter. The results will help to modify the present multiplication procedures. Total bacteria counts in the gametophyte culture medium by use of AODC method was attempted and related results were discussed. |
| Key words: Undaria pinnatifida, Male gametophyte, Nutrient uptake |
