| 摘要: |
| 于1995年4—12月在中国科学院海洋研究所进行微藻除菌及比较除菌与自然带菌微藻生长特点的研究。经平板培养排除霉菌后，利用组合抗生素(青霉素+卡那霉素+链霉素+庆大霉素)获得除菌球等鞭金藻、三角褐指藻及小球藻，对抗生素处理前后的微藻生长特点进行比较研究。结果表明，与未除菌时相比，除菌后的球等鞭金藻及小球藻不易老化(可保持良好悬浮30d以上)；回加细菌于除菌藻，藻细胞下沉附底，说明细菌可促使微藻细胞老化。无维生素时，除菌后的球等鞭金藻生长更差，暗示未除菌的球等鞭金藻培养液内可能存在产(类)维生素细菌。除菌后三角褐指藻细胞形态发生一定变化，回加细菌后藻细胞形态有部分恢复。与未除菌时相比，除菌后三角褐指藻更能耐受高温(如30℃)。另外，某些抗生素能够刺激球等鞭金藻的生长。 |
| 关键词: 微藻除菌 延迟老化 细菌作用 |
| DOI：10.11693/hyhz200006010010 |
| 分类号: |
| 基金项目:农业部重点科研项目合同资助项目，渔95-13-96-06-06号 |
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| MARINE MICROALGAL AXENATION AND COMPARISON OF GROWTH CHARACTERISTICS BETWEEN NATURAL AND AXENIC MARINE MICROALGAE |
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LIN Wei, CHEN Dou, LIU Xiu-yun
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Institute of Oceanology,The Chinese Academy of Sciences,Qingdao,266071
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| Abstract: |
| Study on microalgal axenation and comparison of nonaxenic and axenic marine microalgae in some characteristics of growth was carried out. After mould-free algae strains were obtained by selecting algal colony on the plates (algal nutrients contained) without mould, bacteria-free cultures of three algae (Isochrysis galbana 3011, Phaeodactylum tricornutum 2038 and Chlorella sp. 1061) were produced by initially determining effects of several antibiotics on the algae and the co-occurring bacteria, and then using proper antibiotic mixtures (penicillin, gentamycin, kanamycin and streptomycin, all are 25 U/ml in the mould-free algal cultures) to eliminate associated bacteria. Growth characteristics of antibiotic treated and untreated microalgae were compared. Results showed that axenic Isochrysis galbana 3011 and Chlorella sp. 1061 could maintain good condition for one month (compared with nonaxenic algae 3011 and 1061, which began aging after 10 days culturing), some algae-concomitant bacteria could cause aging of algae 3011 and 1061. Bacteria in nonaxenic algae 3011 can produce vitamin(s) (or analogues) and stimulate growth of the algae. Cell shape changes after Phaeodactylum tricornutum 2038 becomes bacteria free, but the shape returns to normal to some extent if the algae-concomitant bacteria meets the algae again. Axenic algae 2038 can tolerate relatively high environmental temperature (30°C, for example) which inhibits growth of the nonaxenic algae seriously. In addition, some antibiotics can stimulate growth of the algae 3011. |
| Key words: Microalgae axenation, Delayed aging, Bacterial influence |
