摘要: |
采用5 种不同饵料投喂斑节对虾幼虾, 研究各饵料对幼虾的体长、体重及成活率的影响, 实验周期为20 天; 然后用投喂攻毒的方法分别感染WSSV, 观察不同饵料对幼虾WSSV 敏感性的影响,实验周期为12 天。结果表明, 投喂卤虫无节幼体组的幼虾, 其体长、体重增长明显优于其它各组(P<0.01), 随后依次为贝肉组、鱼肉组、人工配合饲料组、虾片组。不同饵料对幼虾WSSV 敏感性的影响也有差异:投喂感染WSSV 后, 卤虫组和鱼肉组成活率最高, 明显高于贝肉组、人工配合饲料组和虾片组(P<0.01)。PCR 检测表明, 感染后全部幼虾个体为病毒阳性。
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关键词: 斑节对虾, 饵料, 生长, WSSV, 影响 |
DOI:10.11693/hyhz200903007007 |
分类号: |
基金项目:科技部科技成果转化项目, 2006GB2E000224 号; 广东省科技计划项目, 2007A0204500001 号; 广东省重大科技兴海项目,B200608A203 号 |
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DIFFERENT DIETS ON GROWTH AND WSSV-RESISTANCE OF PENAEUS MONODON |
HU Xian-De1, SUN Cheng-Bo1, DING Shu-Jun1, WANG Ping2, LI Yi-Jun2, XU Zheng-Sheng1, LI Ting1, XU An-Min2
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1.Fisheries College of Guangdong Ocean University;2.Fisheries College of Guangdong Ocean University, Hainan Changjiang Nanjiang Marine Biotechnology Co., Ltd
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Abstract: |
Five different diets were tested to feed artificially-WSSV (white spot syndrome virus) infected Penaeus monodon in high density, and the body length and body weight before and after 20-day experiment were measured and the survival rate calculated. The result shows that the group fed with Artemia salina nauplii had the best, while that of shrimp flakes the worst, in growth performance among all the groups (P<0.01). The group of shellfish took the second place, and that with shellfish, the third. The fish-feeding group grew faster than the one with artificial feed. By infecting with WSSV on Day 12, an obvious (P<0.05) difference occurred among groups. In addition, the death rate of WSSV-infected group was must higher. The analysis results of semi-quantitive PCR show no WSSV infection before but after the experiment when all showed positive sign of infection.
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Key words: Penaeus monodon, Diets, Growth, WSSV, Effect |