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| 西施舌(Coelomactra antiquata)群体遗传多样性与分化的研究 |
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黎中宝1,2,3, 王展林1,2,3, 张桂玲1,2,3, 陈 锦1,2,3, 赵斌丽1,2,3, 吴 宁1,2,3, 林小云1,2,3
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1.集美大学水产学院;2.集美大学水产生物技术研究所;3.福建省高校水产科学技术与食品安全重点实验室
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| 摘要: |
| 采用不连续垂直板聚丙烯酰胺凝胶电泳技术, 对广西北海、福建漳港和江苏启东3个西施舌自然群体的7个等位酶12个位点进行检测分析。结果表明, 西施舌群体的遗传多样性较高, 平均每位点等位基因数目(A)范围为1.5833—2.0000、平均每位点有效等位基因数目(Ae)范围为1.2850—1.5373、多态位点百分数(P)为33.33%、观察杂合度(Ho)为0.2389—0.3083、期望杂合度(He)为0.1496—0.2041 之间。西施舌群体间有一定的遗传分化, 遗传分化系数(FST)为0.0719, 福建漳港群体与广西北海群体之间的FST为0.0413, 但福建漳港群体和广西北海群体与江苏启东群体之间的FST为0.0505。
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| 关键词: 西施舌, 等位酶, 遗传多样性, 遗传分化 |
| DOI：10.11693/hyhz200903008008 |
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| 基金项目:福建省自然科学基金资助项目, B0640010 号; 福建省高等教育新世纪优秀人才支持计划项目, 闽教科[2006]35 号; 福建省高校水产科学技术与食品安全重点实验室基金, 2007J202 号; 集美大学创新团队基金, 2007A001 号 |
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| GENETIC DIVERSITY AND DIFFERENTIATION OF COELOMACTRA ANTIQUATA (SPENGLER) POPULATION |
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LI Zhong-Bao1,2,3, WANG Zhan-Lin1,2,3, ZHANG Gui-Ling1,2,3, CHEN Jin1,2,3, ZHAO Bin-Li1,2,3, WU Ning1,2,3, LIN Xiao-Yun1,2,3
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1.Fisheries College, Jimei University;2.Institute of Aquaculture Biotechnology, Jimei University;3.Key Laboratory of Aquaculture Technology and Food Safe of Fujian College
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| Abstract: |
| 7 allozymes and 12 loci were detected in 3 natural populations of Coelomactra antiquata (Spengler) from South China using vertical slab polyacrylamide gel electrophoresis. High level of genetic diversity in C. antiquata was found. The mean number of alleles per locus (A) ranged from 1.5833 to 2.0000, and the mean effective number of alleles per locus was from 1.2850 to 1.5373; the percentages of polymorphic loci (P) was 33.33%, the observed heterozygosities (Ho) was from 0.2389 to 0.3083, the expected heterozygosities was from 0.1496 and 0.2041. Genetic differentiation among C. antiquata populations was obvious for having FST at 0.0719, genetic distances among populations ranged from 0.0164 to 0.0302, in average of 0.0245.
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| Key words: Coelmomactra antiquata, Allozyme, Genetic diversity, Genetic differentiation |