| 摘要: |
| 以中华鳖4 个种群80 个个体(太湖种群、日本品系种群、台湾引进种群和“清溪乌鳖”种群)肌肉基因组DNA为模板, 利用已知酪氨酸酶(Tyrosinase, TYR)基因部分序列设计合成特异引物进行PCR 扩增, 克隆并测定了TYR 基因的核苷酸序列。扩增所得的696bp 序列中共有13 个多态性核苷酸位点。中华鳖体色变异种群的3 个个体均在第186 核苷酸位点出现相同的变异。以此设计酶切位点, 选用SmaI 内切酶进行RFLP, 结果显示80 个中华鳖个体的TYR 基因存在多态性。AA 型基因除在中华鳖体色变异种群内未发现外, 其余三个种群内的频率大小顺序为日本品系种群(60%)>台湾引进种群(30%)>太湖种群(20%), 以省外品种为丰富; AB 基因型频率大小顺序为“清溪乌鳖”种群(70%)>台湾引进种群(50%)>太湖种群(40%)>日本品系种群(30%)。
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| 关键词: 中华鳖, 酪氨酸酶基因, RFLP |
| DOI：10.11693/hyhz200903017017 |
| 分类号: |
| 基金项目:浙江省重大科技招标项目《有机蔬菜、水产品安全生产技术集成与标准制定及检测技术研究》, 2007C02002-2 号; 财政部现代农业生产发展资金项目(淡水主导品种选育、推广示范与配套设施建设) |
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| CLONE OF TYR GENE AND POLYMORPHISM IN POPULATIONS OF PELODISCUS SINENSIS |
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ZHANG Yong-Zheng1, ZHANG Hai-Qi2, HUANG Li-Ying3, DING Shi-Hua3
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1.Xiaoshan College of Science & Technology, Zhejiang Ocean University;2.Zhejiang Fisheries Introduction and Breeding Center;3.Lab of Aquatic Bioengineering, Southwest University
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| Abstract: |
| Tyrosinase (TYR) is an essential enzyme in melanogenesis. Genetic DNAs of four Pelodiscus sinensis populations: Taihu Lake population, Japanese breed population, Taiwan-introduced population and Qingxi black mutant population, were prepared as templates from muscle tissue. TYR gene was amplified by PCR with a pair of specific primers designed on the known TYR sequences. 13 nucleotide polymorphisms were detected in the PCR-amplified 696bp fragments. Only one mutation site at nucleotide 186 was identified in three individuals of black mutant of P. sinensis. TYR gene polymorphisms were detected in 80 individuals of P. sinensis using RFLP-PCR analysis with SmaI enzyme. AA haplotype did not appear in the black mutant population but was rich in the Taiwan and Japanese populations. The percentage of AA haplotype was: Japanese breed population (60%) > Taiwan-introduced population (30%) > Taihu Lake population (20%). The percentage of AB haplotype was: Qingxi black mutant population (70%) > Taiwan-introduced population (50%) > Taihu Lake population (40%) > Japanese breed population (30%). These data may provide basic information for molecular breeding of P. sinensis in the future.
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| Key words: Pelodiscus sinensis, Tyrosinase gene, RFLP |
