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| 悬浮物对半滑舌鳎(Cynoglossus semilaevis)幼鱼肝脏溶菌酶、超氧化物歧化酶和鳃丝Na+-K+-ATPase活力的影响 |
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周 勇1, 马绍赛2, 曲克明2, 傅雪军3, 徐 勇2
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1.农业部海洋渔业资源可持续利用重点开放实验室 中国水产科学研究院黄海水产研究所, 上海海洋大学水产与生命学院;2.农业部海洋渔业资源可持续利用重点开放实验室 中国水产科学研究院黄海水产研究所;3.农业部海洋渔业资源可持续利用重点开放实验室 中国水产科学研究院黄海水产研究所,上海海洋大学水产与生命学院
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| 摘要: |
| 在实验室条件下构建不同的悬浮物浓度环境, 采用试剂盒分析方法研究了不同浓度悬浮物对半滑舌鳎幼鱼肝脏溶菌酶(LSZ)、超氧化物歧化酶(SOD)和鳃丝Na+-K+-ATPase 活力的影响。养殖水体中悬浮物浓度添加量分别为0(对照)、50、100、200 和400mg/L, 每5 天采样测定一次, 实验周期为25 天。结果表明, 当悬浮物浓度添加量为50 和100mg/L 时, 肝脏LSZ 和SOD 活力与对照组无显著差异(P>0.05), 当添加量为200 和400mg/L 时, 肝脏LSZ 和SOD 活力与对照组有显著差异(P<0.05); 实验后期各实验组鳃丝Na+-K+-ATPase 活力与对照组均有显著差异(P<0.05)。在悬浮物效应的25 天内, 各实验组肝脏LSZ 活力呈峰值变化, 10 天时除100mg/L 浓度添加组外均达到最大值;而肝脏SOD 活力在50 和100mg/L 浓度添加组略有升高, 在 200 和400mg/L 浓度添加组则基本呈下降趋势; 鳃丝Na+-K+-ATPase 活力前10 天各实验组较对照组无显著变化, 之后则显著降低, 后期基本趋于稳定, 其活力低于初始水平; 实验结束时, 各实验组其肝脏LSZ、SOD 和鳃丝Na+-K+-ATPase活力相对于对照组都受到了不同程度的抑制, 其中各实验组鳃丝Na+-K+-ATPase 活力抑制率分别达到了27.6%、65.2%、57.0%和71.1%。
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| 关键词: 悬浮物, 半滑舌鳎, 溶菌酶, 超氧化物歧化酶, Na+-K+-ATPase |
| DOI：10.11693/hyhz200903018018 |
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| 基金项目:国家科技支撑计划项目, 2006BAD09A03 号; 中央级公益性科研院所基本科研业务费专项资金项目, 2008-gy-04 号。 |
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| EFFECTS OF SUSPENDED SUBSTANCE CONCENTRATION ON ACTIVITIES OF LYSOZYME, SUPEROXIDE DISMUTASE, AND Na+-K+-ATPase IN JUVENILE CYNOGLOSSUS SEMILAEVIS |
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ZHOU Yong,MA Shao-Sai,QU Ke-Ming,FU Xue-Jun,XU Yong
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1.Key Laboratory for Sustainable Utilization of Marine Fisheries Resources, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences;2.College of Fisheries and Life Science,Shanghai Ocean University
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| Abstract: |
| Changes in activities of lysozyme (LSZ), superoxide dismutase (SOD), and Na+-K+-ATPase in the liver and gill of juvenile Cynoglossus semilaevis Günther were studied in different concentrations of suspended substances (0, 50, 100, 200 and 400mg/L). The experiment was conducted in 80L plastic tanks (60cm×40cm×40cm in dimension), each containing 30 fish. The sizes and weights of the fish were at (8.09±0.46)cm and (4.98±0.64)g, respectively. The experimental condition was pH 8.15±0.04, salinity 30.68±0.16, temperature (20.9±0.63)℃, DO (7.03±0.33)mg/L, NH4-N (0.085±0.009)μmol/L, NO2-N (0.155±0.027)μmol/L, and NO3-N (8.56±0.605)μmol/L. Daily bait was fed at 3%—4% of the body weight. The suspended substance used as the experimental mud was taken from a local muddy beach, dried, grinded, and sieved by 400-mech (≤38μm), and the concentration was set at 25.5 (the control), 50, 100, 200, and 400mg/L. Sampling was conducted at Days 0, 5, 10, 15, 20 and 25, and the samples were stored at ?80℃ until enzymes were assayed. The results show that the effects of suspended substance were significant (P<0.05), especially at high concentration (200 and 400mg/L), on the activities of liver LSZ and SOD, gill Na+-K+-ATPase in juvenile C. semilaevis. During the 25 days, the liver LSZ activity increased at first and then decreased, on the Day 10, the peak values appeared in the groups of 50, 200 and 400mg/L, and the minimum ones appeared on Days 20 and 25; both liver SOD and gill Na+-K+-ATPase activities decreased gradually. At the end of the experiment, activities of all the enzymes in each group stabilized. Compared with the control group, the inhibition rate of activities of the three enzymes increased gradually with the increase in the concentration of suspended substance, and the maximum values of liver LSZ increased 35.3% at 200mg/L, liver SOD 33.7% and gill Na+-K+-ATPase at 400mg/L 71.1%, respectively.
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| Key words: Suspended substances, Cynoglossus semilaevis, LSZ, SOD, Na+-K+-ATPase |
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