| 摘要: |
| 采用固体培养基体外抑菌法, 在不同浓度, 相同pH 条件下, 研究了采自浙江秀山海泥制备的不同浓度的海泥水溶体系对大肠杆菌、金黄色葡萄球菌、黑曲霉菌和白色念珠菌的抑制效果及其最低抑菌浓度, 同时与目前公认较好的合成防腐剂对羟基苯甲酸甲酯、对羟基苯甲酸丙酯进行对比,考察了海泥水溶体系的抑菌动力学曲线。结果表明, 浙江秀山滩涂海泥对各种指示菌都有一定程度的抑制作用, 且抑制作用随浓度的增大而增强, 海泥水溶体系对大肠杆菌、金黄色葡萄球菌、黑曲霉菌和白色念珠菌的最低抑菌浓度(MIC)值分别为0.7
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| 关键词: 海泥水溶体系, MIC 值, 抑菌动力学曲线, 抑菌作用 |
| DOI：10.11693/hyhz200905020020 |
| 分类号: |
| 基金项目:浙江省科技厅重点资助项目, 2006C23048 号。 |
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| THE ANTIBACTERIA EFFECTS OF WATER-SOLUBLE PHASE OF SEA MUD |
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YANG Tao, XU Qing, XIN Jian-Mei, LUO Hong-Yu
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Faculty of Food and Pharmacy, Zhejiang Ocean University
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| Abstract: |
| Sea muds taken off Xiushan, Zhejiang were studied for examining in vitro inhibition effect on Escherichia coli, St. aureus, Aspergillus niger and Candida albicansa. The muds in different concentrations at the same pH value were sampled in different places. With well-accepted synthesized antiseptics on hydroxymethyl benzoate and propyl p-hydroxybenzoate, bacteriostasis kinetic curve of soluble phase of sea mud system was established. The results show that the sea muds show a similar antibacterial effect without significant difference (P>0.05). They inhibited bacteria to some degrees, which increased with the increase in sea mud concentration. The minimum inhibition concentration (MIC) of a sea mud on Escherichia coli, St. aureus, Aspergillus niger, and Candida albicansa is 0.75, 1.00, 0.50, and 2.00, respectively. Compared with hydrobenzoate, the inhibition effect of water-soluble phase is similar to that of propyl p-hydroxybenzoate but better. Compared with hydroxymethyl benzoate and propyl p-hydroxybenzoate, the inhibition effect on mold is similar; on yeast is weaker. Bacteriostasis kinetic curve shows that a sea mud containing medium (within 72h after inoculation) depressed the growth of test strains to a lower level in 100% coverage. Therefore, antibacterial application of sea mud shall be promising in food preservation, cosmetic industry and medical field.
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| Key words: Water-soluble system of sea mud, MIC value, Bacteriostasis kinetic curve, Antibacterial effect |
