| 摘要: |
| 采用DGGE 技术对北黄海和青岛近海藻类DNA 病毒群落多样性进行调查。结果表明, 在北黄海A604 站、B107 站和C803 站分别得到10 个、8 个、8 个OTUs, 在青岛近海ZQ 站得到6 个OTUs, 说明不同海域藻类DNA 病毒多样性不同, 在北黄海较丰富, 青岛近海较低。北黄海3 个站间相比, OTUs 数量和位置均存在差异, 表明同一海域不同站位藻类DNA 病毒多样性也存在差异。在所得OTUs 中, 有2 个OTUs 在4 个站中均存在, 说明在北黄海和青岛近海存在着一些共同的藻类DNA 病毒。4 个站点藻类DNA 病毒优势种均不相同, 在A604 站和ZQ 仅有一个优势种, 而在B107和C803 各有两个优势种。
|
| 关键词: DGGE, 北黄海, 青岛近海, 藻类DNA 病毒, 遗传多样性 |
| DOI：10.11693/hyhz201004008008 |
| 分类号: |
| 基金项目:国家908 专项ST02 区块水体环境调查与研究项目资助, 2006—2008; 国家海洋局公益项目资助, 2008—2011 |
|
| GENETIC DIVERSITY ANALYSIS OF ALGAE DNA VIRUSES IN THE NORTH YELLOW SEA AND QINGDAO COASTAL WATERS BY DENATURING GRADIENT GEL ELECTROPHORESIS |
|
WANG Fang, WANG Min, BAI Xiao-Ge, LIANG Yan-Tao, YAN Qun, YANG Lin, LIU Guan-Qun
|
|
Key Laboratory of Marine Genetics and Gene Resource Exploitation, Ocean University of China
|
| Abstract: |
| Algae DNA viruses are abundant and widespread components in oceans, and play important roles in marine ecosystems. The genetic diversity of algae DNA viruses in the North Yellow Sea and Qingdao coastal waters was analysed by denaturing gradient gel electrophoresis (DGGE). The operational taxonomic units (OTUs) were 10, 8, 8, and 6 for aglae DNA viruses collected from the North Yellow Sea at stations of A604, B107 and C803, and from Qingdao coastal waters at station ZQ, respectively. Algal viruses in different waters manifested different degrees of diversity, the degrees of diversity at the North Yellow Sea sampling stations being different among themselves, and being higher than that at the Qingdao coastal waters sampling station. In addition, two similar OTUs were found at all sampling stations, suggesting that certain genetically identical algal viruses have a ubiquitous distribution across these waters. DGGE map indicated that there was one prominent algal virus at stations A604 and ZQ, and two dominant algal viruses at stations B107 and C803.
|
| Key words: DGGE, The North Yellow Sea, Qingdao Coastal Waters, Algae DNA virus, Genetic diversity |
